Plaza Jose A, Bonneau Peter, Prieto Victor, Sangueza Martin, Mackinnon Alexander, Suster David, Bacchi Carlos, Estrozi Bruna, Kazakov Dmitry, Kacerovska Denisa, Falconieri Giovanni, Suster Saul
Dermatopathology, Medical College of Wisconsin, Milwaukee, WI, USA.
Pathology, Medical College of Wisconsin, Milwaukee, WI, USA.
J Cutan Pathol. 2016 Apr;43(4):313-23. doi: 10.1111/cup.12654. Epub 2016 Jan 27.
Desmoplastic melanoma (DM) is histologically characterized by a proliferation of spindle melanocytes dispersed in a collagenous stroma that can be mistaken for a variety of neoplasms. The purpose of this study was to analyze 40 cases of DM with a comprehensive panel of immunohistochemical markers (KBA.62, p16, Ezrin, WT-1, MITF-1, SOX-10, CD117, SOX-2, nestin, PNL2, p75, MART-1, gp100 and S100p) to obtain a more complete understanding of the potential use of these antibodies in the diagnosis of DM. We found that all cases of DM expressed p16, WT-1, SOX-10, nestin and S100p and 95% of cases expressed p75. There was variable expression with Ezrin, SOX-2, KBA.62, MART-1 and HMB-45. Most DMs did not express MITF-1, PNL2 and CD117. Conditions that may enter in the histologic differential diagnosis of DM, including dermal scars, fibromatosis and dermatofibromas were also studied. Nearly all control cases also stained positive for p16 but were negative for WT1, SOX10, nestin, p75 and S-100p, as well as for most of the other markers tested. We conclude that a panel of S-100p, WT1, SOX10, p75 and nestin may constitute the optimal panel with the most sensitive and specific combination of immunostain available for the diagnosis of DM.
促纤维增生性黑色素瘤(DM)在组织学上的特征是梭形黑素细胞在胶原基质中增生,这可能会被误诊为多种肿瘤。本研究的目的是用一组全面的免疫组化标志物(KBA.62、p16、埃兹蛋白、WT-1、MITF-1、SOX-10、CD117、SOX-2、巢蛋白、PNL2、p75、MART-1、gp100和S100p)分析40例DM,以更全面地了解这些抗体在DM诊断中的潜在用途。我们发现,所有DM病例均表达p16、WT-1、SOX-10、巢蛋白和S100p,95%的病例表达p75。埃兹蛋白、SOX-2、KBA.62、MART-1和HMB-45存在可变表达。大多数DM不表达MITF-1、PNL2和CD117。还研究了可能参与DM组织学鉴别诊断的情况,包括皮肤瘢痕、纤维瘤病和皮肤纤维瘤。几乎所有对照病例p16染色也呈阳性,但WT1、SOX10、巢蛋白、p75和S-100p以及大多数其他检测标志物均为阴性。我们得出结论,一组S-100p、WT1、SOX10、p75和巢蛋白可能构成用于DM诊断的最敏感和特异免疫染色组合的最佳标志物组。