Guidotti Stefano, Verstappen-Dumoulin Berthe M A A A, Jansen Henk G, Aerts-Bijma Anita T, van Vliet André A, Scheurink Anton J W, Meijer Harro A J, van Dijk Gertjan
Center for Isotope Research (CIO), Energy and Sustainability Research Institute Groningen (ERSIG), University of Groningen, Nijenborgh 4, 9747, AG, Groningen, The Netherlands.
Groningen Institute for Evolutionary Life Sciences (GELIFES), Unit Behavioral Neuroscience, University of Groningen, Nijenborgh 7, 9747, AG, Groningen, The Netherlands.
Rapid Commun Mass Spectrom. 2016 Jan 15;30(1):143-50. doi: 10.1002/rcm.7412.
The doubly labelled water (DLW) method is a stable isotopic technique for measuring total energy expenditure (TEE). Saliva is the easiest sampling fluid for assessing isotopic enrichments, but blood is considered superior because of its rapid exchange with body water. Therefore, we compared a large range of isotopic enrichments in saliva and blood, and related TEE in subjects with their ad libitum total energy intake (TEI). The relevance of these parameters to body weight and fat change over an 8-day interval was also assessed.
Thirty subjects underwent DLW analysis over either 8 or 14 days, during which time initial and final blood and saliva enrichments were compared. TEI was assessed by dieticians over the 8-day period only. Isotope ratio mass spectrometry was used for the measurement of δ(2)H and δ(18)O values.
No discrepancies were observed between sampling fluids over a wide range of enrichments. During the 8-day period, average TEI exceeded TEE by ~5% or less. Using saliva as sampling fluid, TEI and TEI-TEE, but not TEE, were positively correlated to body weight change. TEI-TEE and physical activity EE (AEE), but not TEI, correlated, respectively, positively and negatively to changes in fat mass.
The DLW method in humans can be reliably applied using saliva as sampling fluid. TEI-TEE as well as AEE contributes significantly to changes in fat mass over an 8-day period.
双标记水（DLW）法是一种用于测量总能量消耗（TEE）的稳定同位素技术。唾液是评估同位素富集情况最容易采集的液体样本，但血液因其与身体水分的快速交换而被认为更具优势。因此，我们比较了唾液和血液中一系列同位素富集情况，并将受试者的TEE与其自由摄入的总能量摄入量（TEI）相关联。还评估了这些参数在8天时间间隔内与体重和脂肪变化的相关性。
30名受试者在8天或14天内接受了DLW分析，在此期间比较了初始和最终的血液及唾液富集情况。仅由营养师在8天期间评估TEI。使用同位素比率质谱法测量δ(2)H和δ(18)O值。
在广泛的富集范围内，未观察到样本液体之间存在差异。在8天期间，平均TEI超过TEE约5%或更少。以唾液作为样本液体时，TEI和TEI - TEE与体重变化呈正相关，但TEE与体重变化无此相关性。TEI - TEE和身体活动能量消耗（AEE）分别与脂肪量变化呈正相关和负相关，但TEI与脂肪量变化无此相关性。
在人体中，DLW法可以可靠地使用唾液作为样本液体来应用。在8天期间，TEI - TEE以及AEE对脂肪量变化有显著贡献。
