维生素C对源自人脐带血的血红蛋白基氧载体的抗氧化作用。
Antioxidant effects of vitamin C on hemoglobin-based oxygen carriers derived from human cord blood.
作者信息
Chen Gang, Duan Yingying, Liu Jiaxin, Wang Hong, Yang Chengmin
机构信息
a Institute of Blood Transfusion, Chinese Academy of Medical Science , Chengdu , P. R. China ;
b Institute of Tianjin Union Biological Science Development , Tianjin , P. R. China.
出版信息
Artif Cells Nanomed Biotechnol. 2016;44(1):56-61. doi: 10.3109/21691401.2015.1111239. Epub 2015 Dec 15.
OBJECTIVE
To investigated the antioxidant protection effects of vitamin C (Vc) on the hemoglobin-based oxygen carriers (HBOCs) solution.
METHODS
Three influential variables were studied including the molar ratio of Vc and hemoglobin, pH and temperature. The HBOCs molecules were characterized through spectral analysis, circular dichroism spectra, the oxygen binding curves and the molecular weights.
RESULTS
The optimized conditions were molar ratios of above 3, pH of 7.5-8.0 and temperature of 4 °C. HBOCs characterization showed no differences before and after Vc addition.
CONCLUSION
Antioxidant effects in HBOCs solution could be presented by Vc, indicating the potential application of Vc in HBOCs development.
目的
研究维生素C（Vc）对基于血红蛋白的氧载体（HBOCs）溶液的抗氧化保护作用。
方法
研究了三个影响因素，包括Vc与血红蛋白的摩尔比、pH值和温度。通过光谱分析、圆二色光谱、氧结合曲线和分子量对HBOCs分子进行表征。
结果
优化条件为摩尔比大于3、pH值为7.5 - 8.0、温度为4℃。添加Vc前后HBOCs的表征无差异。
结论
Vc可在HBOCs溶液中发挥抗氧化作用，表明Vc在HBOCs开发中的潜在应用价值。
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