Zhang Bao, Li Xuan-zhen, Feng Fa-jie, Gu Li, Zhang Jun-yi, Zhang Liu-ji, Zhang Zhong-yi
Zhong Yao Cai. 2015 Apr;38(4):659-63.
To study the allelopathic potential of Rehmannia glutinosa root exudates in different growth stages and dynamic change of phenolic acids contents, in order to reveal the correlation between phenolic acids and allelbpathy effect of Rehmannia glutinosa.
Root exudates of Rehmannia glutinosa in different growth stages were obtained by a new instrument which was used to collect the root exudates of xerophytes. After that, bioassay was applied to estimate allelopathy effect of the root exudates. HPLC was used to determine the contents of five phenolic acids (coumaric acid, 4-hydroxybenzoic acid, vanillic acid, syringic acid and ferulic acid) which were reported to relate to allelopathy effect. Correlation of bioassay data and HPLC data were also analyzed.
The germination rate of radish after soaking by root exudates of different growth stages of Rehmannia glutinosa was 97. 89%, 92. 38%, 89. 52%, 85. 71%, 85. 71%, 84. 76% and 83. 81%, respectively, which indicated a decline trend. And significant differences were shown from previous enlargement stage compared with the contrast. The bud length after soaking by root exudates was 5. 68, 5. 76, 5. 91, 5. 65, 5. 41, 5. 28 and 5. 11 cm, separately, which increased slightly before decreasing gradually. Previous enlargement stage was also the initial period when significant differences were shown. Five phenolic acids were detected in root exudates by HPLC, while the change of their contents and the allelopathy effect of root exudates did not perform a similar trend. Correlation analysis indicated the five phenolic acids did not have significant relevance (r = - 0. 666 - 0. 590) with germination rate and bud length of radish except the negative correlation (r = -0. 833, P <0. 05) of syringic acid and bud length.
Significant allelopathy effect of Rehmannia glutinosa is performed from previous enlargement stage and enhanced with its growth. Syringic acid is a probable dominant allelochemical of Rehmannia glutinosa.
研究不同生长时期地黄根系分泌物的化感潜力及酚酸类物质含量的动态变化，以揭示酚酸类物质与地黄化感效应之间的相关性。
采用一种用于收集旱生植物根系分泌物的新仪器获取不同生长时期地黄的根系分泌物，之后通过生物测定法评估根系分泌物的化感效应。采用高效液相色谱法测定5种与化感效应相关的酚酸（香豆酸、4-羟基苯甲酸、香草酸、丁香酸和阿魏酸）的含量，并分析生物测定数据与高效液相色谱数据的相关性。
不同生长时期地黄根系分泌物浸泡后的萝卜种子发芽率分别为97.89%、92.38%、89.52%、85.71%、85.71%、84.76%和83.81%，呈下降趋势，且从膨大前期开始与对照相比差异显著。根系分泌物浸泡后的芽长分别为5.68、5.76、5.91、5.65、5.41、5.28和5.11 cm，先略有增加后逐渐下降，膨大前期也是差异显著的起始时期。高效液相色谱法检测到根系分泌物中含有5种酚酸，但其含量变化与根系分泌物的化感效应未呈现相似趋势。相关性分析表明，除丁香酸与芽长呈负相关（r = -0.833，P <0.05）外，这5种酚酸与萝卜种子发芽率和芽长均无显著相关性（r = -0.�66 - 0.590）。
地黄从膨大前期开始表现出显著的化感效应，并随着生长而增强。丁香酸可能是地黄的主要化感物质。
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