Yang Chuyun, Xie Zhuomi, Qian Sheng, Zhang Junyi, Yu Zhijian, Li Mingjie, Gu Li, Qin Shuangshuang, Zhang Zhongyi
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China.
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Plant Sci. 2022 Oct 31;13:1044070. doi: 10.3389/fpls.2022.1044070. eCollection 2022.
Previous studies have indicated that some Leucine-rich repeat receptor-like protein kinases (LRR-RLKs) are involved in the formation of replant disease. However, it remains unclear how the interaction of LRR-RLKs with a key factor, the interaction between root exudates and , results in formation of replant disease. In this study, the influences of root exudates, and the interaction of these two factors on expression of nine () were analyzed. The resulting eight of them were highly expressed at the early stage, and rapidly declined at later stages under mixed treatment of root exudates and . The functions of nine under root exudates, and mixed treatment of root exudates and were preliminarily analyzed using transient overexpression and RNAi experiments. The results showed that high expression of , , and could decrease the damage to root cells from the mixed treatment of root exudates and , but the interference of these genes enhanced the damage levels of root cells. Based on this, stable transgenic seedlings were acquired. Overexpression of conferred resistance of seedlings to root exudates, and mixed treatment. These results indicated that the continuous proliferation of supported by root exudates altered the expression patterns of in , then disordered the growth and development of , finally leading to the formation of replant disease.
先前的研究表明，一些富含亮氨酸重复序列的类受体蛋白激酶（LRR-RLKs）参与了连作障碍的形成。然而，LRR-RLKs与一个关键因素（根系分泌物与……之间的相互作用）如何导致连作障碍的形成仍不清楚。在本研究中，分析了根系分泌物、……以及这两个因素的相互作用对九个……（）表达的影响。其中八个在早期高表达，在根系分泌物和……混合处理下后期迅速下降。利用瞬时过表达和RNA干扰实验初步分析了九个……在根系分泌物、……以及根系分泌物和……混合处理下的功能。结果表明，……、……、……和……的高表达可降低根系分泌物和……混合处理对根细胞的损伤，但这些基因的干扰增强了根细胞的损伤水平。基于此，获得了稳定的转基因……幼苗。……的过表达赋予了……幼苗对根系分泌物、……和混合处理的抗性。这些结果表明，根系分泌物支持的……持续增殖改变了……中……的表达模式，进而扰乱了……的生长发育，最终导致连作障碍的形成。
