Functional analysis of key LRR-RLKs during interaction of root exudates with reveals the roles of immune proteins in formation of replant disease.

Previous studies have indicated that some Leucine-rich repeat receptor-like protein kinases (LRR-RLKs) are involved in the formation of replant disease. However, it remains unclear how the interaction of LRR-RLKs with a key factor, the interaction between root exudates and , results in formation of replant disease. In this study, the influences of root exudates, and the interaction of these two factors on expression of nine () were analyzed. The resulting eight of them were highly expressed at the early stage, and rapidly declined at later stages under mixed treatment of root exudates and . The functions of nine under root exudates, and mixed treatment of root exudates and were preliminarily analyzed using transient overexpression and RNAi experiments. The results showed that high expression of , , and could decrease the damage to root cells from the mixed treatment of root exudates and , but the interference of these genes enhanced the damage levels of root cells. Based on this, stable transgenic seedlings were acquired. Overexpression of conferred resistance of seedlings to root exudates, and mixed treatment. These results indicated that the continuous proliferation of supported by root exudates altered the expression patterns of in , then disordered the growth and development of , finally leading to the formation of replant disease.