Embery G, Rolla G
Scand J Dent Res. 1977 May;85(4):241-6. doi: 10.1111/j.1600-0722.1977.tb00559.x.
A 35S-labeled sulfated glycoprotein was isolated from rabbit submandibular glands. Acid stability studies on the 35sulfate groupings present in the intact glycoprotein gave a half-life of 45 min. Partial acid hydrolysis of the 35S-labeled glycoprotein in 0.1 M HC1 for 90 min at 100 degrees C liberated a radioactive fraction which was free from peptide and fractionated in the monosaccharide range of a Sephadex G-15 column. Examination of this fraction by paper chromatography revealed the presence of a major component having the characteristics of N-acetylglucosamine 6-0-sulfate and a minor component having the properties of N-acetylgalactosamine 6-0-sulfate. The presence of ester sulfate groups in the intact glycoprotein was confirmed by infrared spectroscopy.
从兔下颌下腺中分离出一种35S标记的硫酸化糖蛋白。对完整糖蛋白中存在的35硫酸基团进行酸稳定性研究，得出半衰期为45分钟。将35S标记的糖蛋白在0.1M盐酸中于100℃下进行90分钟的部分酸水解，释放出一个不含肽的放射性部分，该部分在Sephadex G - 15柱的单糖范围内进行分级分离。通过纸色谱法对该部分进行检测，发现存在一种具有N - 乙酰葡糖胺6 - O - 硫酸酯特征的主要成分和一种具有N - 乙酰半乳糖胺6 - O - 硫酸酯性质的次要成分。通过红外光谱法证实了完整糖蛋白中存在硫酸酯基团。
