Guo Yeqing, Liu Jia, Cao Wuzhan, Deng Li, Yao Sui
Department of Obstetrics, Hunan Provincial People's Hospital, Changsha 410005, China.
Department of Obstetrics, Hunan Provincial People's Hospital, Changsha 410005, China; Email:
Zhonghua Fu Chan Ke Za Zhi. 2015 Oct;50(10):747-51.
To investigate the role of angiogenic T cells (Tang) and endothelial progenitor cells (EPC) in the pathogenesis of preeclampsia. To explore the relationship between Tang and EPC.
From Mar 2013 to Aug 2014, 40 patients diagnosed preeclampsia (PE) and delivered in Hunan Provincial People's Hospital. A total of 20 of them were defined as the mild preeclampsia group and the other 20 cases were recruited as the severe preeclampsia group. And 24 healthy pregnant women were recruited as the control group. The percentage of Tang and EPC in peripheral blood mononuclear cells (PBMC) were determinated by flow cytometry between 28 and 40 gestational weeks.
(1) There was no significant difference in the age, pre-pregnancy body mass index (Pre-BMI) or gestational age among the three groups (P > 0.05). The differences of blood pressure among the three groups were statistically significant (P < 0.05). The gestational week at delivery, the birthweight of the neonates and the 1 minute Apgar score in the severe preeclampsia group were lower than those in the mild preeclampsia group and the control group, with statistically significant differences (P < 0.05). The morbidity of neonatal asphyxia in the severe preeclampsia group was 35% (7/20); and in the mild preeclampsia group it was 5% (1/20), with statistically significant difference (P < 0.05). (2) The percentage of Tang in maternal peripheral blood was (52.7 ± 8.0)%, (47.5 ± 8.8)% and (45.5 ± 8.7)% in the control group, the mild preeclampsia group and the severe preeclampsia group, respectively. The difference among the three groups was significant (F = 4.248, P < 0.05), and SNK q analysis showed there was significant difference between the control group and the severe preeclampsia group (P < 0.05). While there was no statistically significant difference between the mild and the severe preeclampsia group, nor between the control group and the mild preeclampsia group(P > 0.05). (3) The percentage of EPC in maternal peripheral blood was (0.16 ± 0.07)%, (0.09 ± 0.07)% and (0.08 ± 0.05)% in the control group, the mild and the severe preeclampsia group, respectively. Analysis of variance showed that difference among the three groups was significant (F = 9.351, P < 0.05). The percentage of EPC in the mild or the severe preeclampsia group was significantly higher than that of the control group (P < 0.05). (4) There was no statistically significant correlation between the Tang level and the EPC level in the control group (r = -0.325, P > 0.05). In the preeclampsia group (including mild and severe cases), there was positive correlation between the Tang level and EPC level (r = 0.667, P < 0.01). The positive correlation between Tang level and EPC level were proved respectively in the mild preeclampsia group (r = 0.803, P < 0.01) and the severe preeclampsia group (r = 0.520, P < 0.05).
The number of Tang had some correlation with the pathogenesis of preeclampsia. The percentage of Tang had positive correlation with the level of EPC in women with preeclampsia. Tang might have some influence on the change of EPC' level. Tang together with EPC were likely to contribute to the angiogenesis in preeclampsia.
探讨血管生成性T细胞（Tang）和内皮祖细胞（EPC）在子痫前期发病机制中的作用。探究Tang与EPC之间的关系。
2013年3月至2014年8月，40例在湖南省人民医院诊断为子痫前期（PE）并分娩的患者。其中20例被定义为轻度子痫前期组，另外20例被纳入重度子痫前期组。24例健康孕妇作为对照组。在妊娠28至40周期间，通过流式细胞术测定外周血单个核细胞（PBMC）中Tang和EPC的百分比。
（1）三组患者的年龄、孕前体重指数（Pre-BMI）或孕周差异无统计学意义（P>0.05）。三组患者血压差异有统计学意义（P<0.05）。重度子痫前期组的分娩孕周、新生儿出生体重和1分钟Apgar评分低于轻度子痫前期组和对照组，差异有统计学意义（P<0.05）。重度子痫前期组新生儿窒息发生率为35%（7/20）；轻度子痫前期组为5%（1/20），差异有统计学意义（P<0.05）。（2）对照组、轻度子痫前期组和重度子痫前期组母体外周血中Tang的百分比分别为（52.7±8.0）%、（47.5±8.8）%和（45.5±8.7）%。三组间差异有统计学意义（F=4.248，P<0.05），SNK q检验显示对照组与重度子痫前期组差异有统计学意义（P<0.05）。轻度和重度子痫前期组之间以及对照组与轻度子痫前期组之间差异无统计学意义（P>0.05）。（3）对照组、轻度和重度子痫前期组母体外周血中EPC的百分比分别为（0.16±0.07）%、（0.09±0.07）%和（0.08±0.05）%。方差分析显示三组间差异有统计学意义（F=9.351，P<0.05）。轻度或重度子痫前期组中EPC的百分比显著高于对照组（P<0.05）。（4）对照组中Tang水平与EPC水平无统计学意义的相关性（r=-0.325，P>0.05）。子痫前期组（包括轻度和重度病例）中，Tang水平与EPC水平呈正相关（r=0.667，P<0.01）。在轻度子痫前期组（r=0.803，P<0.01）和重度子痫前期组（r=0.520，P<0.05）中分别证实了Tang水平与EPC水平的正相关。
Tang数量与子痫前期发病机制有一定相关性。子痫前期患者中Tang百分比与EPC水平呈正相关。Tang可能对EPC水平变化有一定影响。Tang与EPC可能共同促成子痫前期的血管生成。
