Alsalleeh F, Williams S, Jaber H
King Saud University, College of Dentistry, Restorative Dental Sciences, Saudi Arabia; Department of Surgical Specialties, University of Nebraska Medical Center, College of Dentistry, USA.
Department of Surgical Specialties, University of Nebraska Medical Center, College of Dentistry, USA.
Arch Oral Biol. 2016 Mar;63:47-52. doi: 10.1016/j.archoralbio.2015.11.012. Epub 2015 Nov 30.
Candida albicans is the most numerous commensal and potentially pathological yeast in the human oral cavity. The purpose herein is to investigate the ability of C. albicans to form a biofilm in the presence of periodontal ligament (PDL) fibroblasts.
Silicone elastomer disks (SE) were transferred to wells containing PDL cells. C. albicans suspension was added to each well. The whole mixed culture was then allowed to form a biofilm for 48h. Biofilms were quantified by tetrazolium-salt-based (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl amino) carbonyl]- 2H-tetrazolium hydroxide (XTT). Furthermore, biofilm was visualized by confocal scanning laser and scanning electron microscopy. Migration of C. albicans and its ability to form biofilms in presence of PDL cells was determined by using a transwell system. Last, elutes obtained from co-culturing C. albicans and PDL cells were added to SE disks and covered with C. albicans. The culture plate was then incubated to allow biofilm formation. Biofilms formed over SE disks were quantified using XTT.
PDL cells significantly limited the biofilm formation at incubation interval of 48h. PDL cells induced less biofilm compared to mature and thick hyphae in the absence of PDL cells as seen in confocal scanning laser and scanning electron microscopy. The presence of PDL cells limited the migration and formation of biofilm by C. albicans. Elutes obtained from co-culturing PDL cells with C. albicans for one hour induced significantly less biofilm.
This is the first study to report that PDL cells exhibit antifungal activity. While the exact mechanism of how PDL cells limited biofilm formation is yet unknown, it was clear that competent PDL cells promote resistance to C. albicans biofilm formation.
白色念珠菌是人类口腔中数量最多的共生且具有潜在致病性的酵母菌。本文旨在研究白色念珠菌在存在牙周膜（PDL）成纤维细胞的情况下形成生物膜的能力。
将硅橡胶弹性体圆盘（SE）转移至含有PDL细胞的孔中。向每个孔中加入白色念珠菌悬液。然后使整个混合培养物形成生物膜48小时。通过基于四氮唑盐的方法（2,3 - 双（2 - 甲氧基 - 4 - 硝基 - 5 - 磺基苯基） - 5 - [（苯基氨基）羰基] - 2H - 四氮唑氢氧化合物（XTT））对生物膜进行定量。此外，通过共聚焦扫描激光显微镜和扫描电子显微镜观察生物膜。使用Transwell系统测定白色念珠菌的迁移及其在存在PDL细胞时形成生物膜的能力。最后，将白色念珠菌与PDL细胞共培养获得的洗脱液添加到SE圆盘上，并覆盖白色念珠菌。然后将培养板孵育以形成生物膜。使用XTT对在SE圆盘上形成的生物膜进行定量。
在48小时的孵育间隔时，PDL细胞显著限制了生物膜的形成。如在共聚焦扫描激光显微镜和扫描电子显微镜中所见，与不存在PDL细胞时的成熟且粗大的菌丝相比，PDL细胞诱导形成的生物膜更少。PDL细胞的存在限制了白色念珠菌的迁移和生物膜的形成。将PDL细胞与白色念珠菌共培养一小时获得的洗脱液诱导形成的生物膜明显更少。
这是第一项报道PDL细胞具有抗真菌活性的研究。虽然PDL细胞如何限制生物膜形成的确切机制尚不清楚，但很明显，有活性的PDL细胞可促进对白色念珠菌生物膜形成的抗性。
