Mumaw Jennifer L, Schmiedt Chad W, Breidling Sarah, Sigmund Alex, Norton Natalie A, Thoreson Merrilee, Peroni John F, Hurley David J
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, 2200 College Station Road, Athens, GA 30602, United States.
Res Vet Sci. 2015 Dec;103:60-9. doi: 10.1016/j.rvsc.2015.09.010. Epub 2015 Sep 14.
Feline bone marrow-derived MSCs (BMMSCs), adipose-derived MSCs (AMSCs) and fibroblasts (FBs) were isolated and cultured. Tri-lineage differentiation assays and flow cytometry were used to characterize MSCs. Neutrophils (NPs) were isolated from whole blood and the NPs production of reactive oxygen reactive oxygen species (ROS) was measured. NPs were cultured alone, with MSC culture supernatant (SN), BMMSCs or AMSCs. NPs incubated with BMMSCs had significantly lower ROS production than NPs incubated with AMSCs (p=0.0006) or FB (p<0.0001); NPs ROS production significantly decreased with increasing BMMSC cell number (p=0.0023) and significantly increased with NPs were incubated with FB compared to BMMSC (p=0.0003). Both BMMSC SN and AMSC SN had statistically significantly lower ROS production than FB SN when incubated with NPs (both p<0.0001). ROS production was significantly reduced with increased fractions of SN from BMMSCs (p=0.0467) and AMSCs (p=0.0017).
分离并培养猫骨髓来源的间充质干细胞（BMMSCs）、脂肪来源的间充质干细胞（AMSCs）和成纤维细胞（FBs）。采用三系分化试验和流式细胞术对间充质干细胞进行鉴定。从全血中分离出中性粒细胞（NPs），并检测NPs产生活性氧（ROS）的情况。NPs单独培养，或与间充质干细胞培养上清液（SN）、BMMSCs或AMSCs共同培养。与AMSCs（p = 0.0006）或FB（p < 0.0001）共同培养的NPs相比，与BMMSCs共同培养的NPs产生的ROS显著降低；随着BMMSC细胞数量增加，NPs的ROS产生显著降低（p = 0.0023），与BMMSC相比，与FB共同培养的NPs的ROS产生显著增加（p = 0.0003）。当与NPs共同培养时，BMMSC SN和AMSC SN产生的ROS均显著低于FB SN（p均< 0.0001）。随着BMMSCs（p = 0.0467）和AMSCs（p = 0.0017）SN比例的增加，ROS产生显著降低。
