环氧合酶-2 蛋白在胃癌发生中的表达。

Expression of Cyclooxygenase-2 Protein in Gastric Carcinogenesis.

出版信息

Cancer Res Treat. 2002 Aug;34(4):252-7. doi: 10.4143/crt.2002.34.4.252.

DOI:10.4143/crt.2002.34.4.252

Abstract

PURPOSE

The increased expression of cyclooxygenase (COX)-2 has been implicated in the development and progression of human cancers. We investigated COX-2 expression in normal, gastric adenomas and adenocarcinomas.

MATERIALS AND METHODS

COX-2 protein was assayed in gastrectomy and biopsy specimens, from 68 gastric adenocarcinomas, 40 gastric adenomas and 35 normal gastric tissues, by immunohistochemistry, and 32 specimens of normal and adenocarcinomas by western blot analysis. Correlation between COX-2 expression and various clinicopathological factors were studied in the gastric adenocarcinoma.

RESULTS

COX-2 protein expression in epithelial cells was increased in 6/40 (15%) of the adenomas and 55/68 (80.9 %) of the adenocarcinomas, while normal mucosa was not expressed. COX-2 expression was increased in differ-entiated gastric carcinomas compared with those in the undifferentiated group (p<0.05). The expression of COX-2 protein was unrelated to tumor size, depth of tumor invasion and the presence of lymphatic or vascular invasions. Western blot analysis showed the enhanced expression of the COX-2 protein (23 out of 32)(71%) in gastric carcinomas compared to that of normal gastric mucosal epithelium.

CONCLUSION

The above results indicated that the expression of COX-2 protein occurs in dysplastic epithelium and gastric carcinomas, which suggests COX-2 expression may contribute to tumor formation.

摘要

目的

环氧化酶-2（COX-2）的表达增加与人类癌症的发生和发展有关。我们研究了 COX-2 在正常、胃腺瘤和腺癌中的表达。

材料和方法

通过免疫组织化学检测了 68 例胃腺癌、40 例胃腺瘤和 35 例正常胃组织中 COX-2 蛋白，并用 Western blot 分析了 32 例正常和腺癌标本。研究了 COX-2 表达与胃腺癌各种临床病理因素的关系。

结果

在 40 例腺瘤中有 6 例（15%）和 68 例腺癌中有 55 例（80.9%）上皮细胞中 COX-2 蛋白表达增加，而正常黏膜不表达。与未分化组相比，分化型胃癌中 COX-2 表达增加（p<0.05）。COX-2 蛋白的表达与肿瘤大小、肿瘤浸润深度以及有无淋巴管或血管浸润无关。Western blot 分析显示，与正常胃黏膜上皮相比，胃癌中 COX-2 蛋白的表达增强（32 例中有 23 例）（71%）。