重金属抗性木糖氧化无色杆菌胞外多糖生物絮凝剂的纯化及特性研究。
Purification and characterization of exopolysaccharide bioflocculant produced by heavy metal resistant Achromobacter xylosoxidans.
机构信息
Environmental and Industrial Biotechnology Division, The Energy and Resources Institute, Lodi Road, New Delhi, India.
Environmental and Industrial Biotechnology Division, The Energy and Resources Institute, Lodi Road, New Delhi, India.
出版信息
Carbohydr Polym. 2016 Feb 10;137:441-451. doi: 10.1016/j.carbpol.2015.10.066. Epub 2015 Oct 27.
Optimization of process parameters enhanced bioflocculating activity of 'Achromobacter xylosoxidans strain TERI L1' from 75% to 83.3% in absence of heavy metals, which decreased to 73% in presence of multi-metals. 'TERI L1' could adsorb 90% of multi-metals when grown in presence of 1250 mg L(-1) Zn, 2 mg L(-1) Cd, 30 mg L(-1) Pb, 200 mg L(-1) Ni and 90 mg L(-1) Cu and could adsorb 1100 mg L(-1) of Pb when grown in presence of 1500 ppm lead nitrate. The bioflocculant was purified and characterized. Bioflocculant yield was 5 g L(-1). Fourier transform infrared spectrum indicated presence of carboxyl, hydroxyl, amino groups, typical of glycoprotein. Spectroscopic analysis of bioflocculant by nuclear magnetic resonance revealed that it is a glycoprotein. LC-MS analysis confirmed the bioflocculant as a carbohydrate hetero polymer. Bioflocculant was composed of 75% total sugar with 72.9% neutral sugar and 11.5% protein. Scanning Electron Micrography revealed effective flocculation of kaolin clay by purified exopolysaccharide bioflocculant.
在没有重金属的情况下，优化工艺参数可将“无色杆菌菌株 TERI L1”的生物絮凝活性从 75%提高到 83.3%，而在多金属存在的情况下则降低到 73%。当在存在 1250 mg/L 的 Zn、2 mg/L 的 Cd、30 mg/L 的 Pb、200 mg/L 的 Ni 和 90 mg/L 的 Cu 的条件下生长时，“TERI L1”可以吸附 90%的多金属，而当在存在 1500 ppm 硝酸铅的条件下生长时，可以吸附 1100 mg/L 的 Pb。生物絮凝剂被纯化和表征。生物絮凝剂的产率为 5 g/L。傅里叶变换红外光谱表明存在羧基、羟基、氨基，典型的糖蛋白。通过核磁共振对生物絮凝剂的光谱分析表明它是一种糖蛋白。LC-MS 分析证实生物絮凝剂是一种碳水化合物杂聚物。生物絮凝剂由 75%的总糖组成，其中 72.9%为中性糖，11.5%为蛋白质。扫描电子显微镜显示，纯化的胞外多糖生物絮凝剂可有效絮凝高岭土粘土。
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