铕标记蛋白A时间分辨荧光免疫分析法测定血清孕酮的评估

Evaluation of time-resolved fluoroimmunoassay with Eu-labelled protein-A for serum progesterone.

作者信息

Ius A, Ferrara L, Meroni G, Bacigalupo M A

机构信息

Istituto di Chimica degli Ormoni, C.N.R., Milano, Italy.

出版信息

J Steroid Biochem. 1989 Jul;33(1):101-3. doi: 10.1016/0022-4731(89)90363-4.

DOI:10.1016/0022-4731(89)90363-4

PMID:2668643

Abstract

Progesterone has been assayed in several serum samples by a time-resolved fluoroimmunoassay (TR-FIA). The solid phase was 11 alpha-hydroxyprogesterone hemisuccinate bound covalently to ovalbumin and adsorbed on wells of polystyrene. The assay was based on competitive reaction of solid phase-bound hormone and samples with specific antibody labelled in situ with protein-A prelabelled with europium. The bound Eu was dissociated from the solid phase by an enhancement solution and measured by time-resolved fluorometry. The coefficient of correlation between TR-FIA and RIA was 0.97.

摘要

已通过时间分辨荧光免疫分析法（TR-FIA）对多个血清样本中的孕酮进行了测定。固相为与卵清蛋白共价结合并吸附在聚苯乙烯孔上的11α-羟基孕酮半琥珀酸酯。该分析基于固相结合的激素和样本与用铕预标记的蛋白A原位标记的特异性抗体之间的竞争反应。结合的铕通过增强溶液从固相上解离，并通过时间分辨荧光法进行测量。TR-FIA与放射免疫分析法（RIA）之间的相关系数为0.97。