Clutterbuck R D, Clarke D, Powles R L, Millar J L
Section of Medicine, Institute of Cancer Research and Royal Marsden Hospital, Surrey, U.K.
Leukemia. 1989 Sep;3(9):637-42.
Normal human bone marrow was grown as xenografts in mice immune-suppressed by thymectomy and total body irradiation. Mononuclear cell fractions isolated from marrow harvests from 17 donors all gave rise to subcutaneous nodules which grew to a variable maximum size and then regressed. Human granulocyte/macrophage progenitors (CFU-GM) were recovered from xenografts up to 20 days postimplantation. Xenograft growth, measured by maximum nodule volume, area under the growth curve, and rate of regression, did not correlate with the speed of neutrophil or platelet recovery in bone marrow transplant patients infused with the same marrow. Assay of numbers of stromal fibroblastoid colony forming cells (CFU-F) in donor marrow was also not predictive of subsequent hemopoietic recovery in recipients. Treatment of host animals with daily intraperitoneal injections of 100 micrograms/kg human recombinant granulocyte/macrophage colony stimulating factor produced a more rapid growth of subcutaneous nodules. This technique may therefore be of use in determining the in vivo efficacy of human hemopoietic regulatory factors.
正常人类骨髓在经胸腺切除和全身照射免疫抑制的小鼠体内作为异种移植物生长。从17名供体的骨髓收获物中分离出的单核细胞部分均产生了皮下结节，这些结节生长到不同的最大尺寸，然后消退。在植入后长达20天的异种移植物中可回收人类粒细胞/巨噬细胞祖细胞（CFU-GM）。通过最大结节体积、生长曲线下面积和消退率测量的异种移植物生长与输注相同骨髓的骨髓移植患者中性粒细胞或血小板恢复的速度无关。对供体骨髓中基质成纤维样集落形成细胞（CFU-F）数量的检测也不能预测受体随后的造血恢复情况。每天腹腔注射100微克/千克人类重组粒细胞/巨噬细胞集落刺激因子对宿主动物进行治疗，可使皮下结节生长更快。因此，该技术可能有助于确定人类造血调节因子的体内疗效。
