Berneman Z N, Chen Z Z, van Bockstaele D, Ramael M, Korthout M, Peetermans M E
Laboratory of Experimental Hematology, University of Antwerp, Antwerp University Hospital, Belgium.
Leukemia. 1989 Sep;3(9):648-61.
An immunological analysis of the nonmacrophage hemopoietic cells in the adherent layer of human long-term bone marrow cultures was performed in situ. It revealed not only the expected granulocytic and monocytic cells, but an important lymphoid population as well. The latter consisted of cells bearing the markers of mature T lymphocytes, B lymphocytes, and plasma cells. These cells were also present in the hemopoietic foci of the adherent layer, the so-called cobblestone areas. Although more CD8+ than CD4+ lymphocytes were generally present in the initial bone marrow inoculum, both the adherent layer and the nonadherent fraction disclosed a preponderance of CD4+ cells after a short period of culture. The majority of the lymphoid cells were present in the adherent layer, rather than in the nonadherent fraction of the cultures. The long-term presence of lymphoid elements in the adherent layer suggests their affinity for the bone marrow stroma and the possible enhancement of their persistence in vitro by contact with these cells.
对人长期骨髓培养物贴壁层中的非巨噬细胞造血细胞进行了原位免疫分析。结果不仅发现了预期的粒细胞和单核细胞，还发现了一个重要的淋巴细胞群体。后者由带有成熟T淋巴细胞、B淋巴细胞和浆细胞标志物的细胞组成。这些细胞也存在于贴壁层的造血灶中，即所谓的卵石区域。虽然在最初的骨髓接种物中通常CD8+淋巴细胞比CD4+淋巴细胞多，但在短期培养后，贴壁层和非贴壁部分都显示出CD4+细胞占优势。大多数淋巴细胞存在于贴壁层，而不是培养物的非贴壁部分。贴壁层中淋巴细胞成分的长期存在表明它们对骨髓基质有亲和力，并且通过与这些细胞接触可能增强它们在体外的持久性。
