印度加尔各答临床分离株中恶性疟原虫K13螺旋桨基因无多态性。
No Polymorphism in Plasmodium falciparum K13 Propeller Gene in Clinical Isolates from Kolkata, India.
作者信息
Chatterjee Moytrey, Ganguly Swagata, Saha Pabitra, Bankura Biswabandhu, Basu Nandita, Das Madhusudan, Guha Subhasish K, Maji Ardhendu K
机构信息
Protozoology Unit, Department of Microbiology, Calcutta School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India.
Protozoology Unit, Department of Microbiology, Calcutta School of Tropical Medicine, 108 Chittaranjan Avenue, Kolkata 700 073, India ; Department of Microbiology, NRS Medical College, 138 AJC Bose Road, Kolkata 700 014, India.
出版信息
J Pathog. 2015;2015:374354. doi: 10.1155/2015/374354. Epub 2015 Nov 25.
Molecular markers associated with artemisinin resistance in Plasmodium falciparum are yet to be well defined. Recent studies showed that polymorphisms in K13 gene are associated with artemisinin resistance. The present study was designed to know the pattern of polymorphisms in propeller region of K13 gene among the clinical isolates collected from urban Kolkata after five years of ACT implementation. We collected 59 clinical isolates from urban Kolkata and sequenced propeller region of K13 gene in 51 isolates successfully. We did not find any mutation in any isolate. All patients responded to the ACT, a combination of artesunate + sulphadoxine-pyrimethamine. The drug regimen is still effective in the study area and there is no sign of emergence of resistance against artemisinin as evidenced by wild genotype of K13 gene in all isolates studied.
恶性疟原虫中与青蒿素抗性相关的分子标记尚未得到明确界定。最近的研究表明，K13基因的多态性与青蒿素抗性有关。本研究旨在了解在采用青蒿琥酯联合磺胺多辛-乙胺嘧啶治疗五年后，从加尔各答市收集的临床分离株中K13基因螺旋桨区域的多态性模式。我们从加尔各答市收集了59株临床分离株，并成功对其中51株分离株的K13基因螺旋桨区域进行了测序。我们在任何分离株中均未发现任何突变。所有患者对青蒿琥酯联合磺胺多辛-乙胺嘧啶的治疗均有反应。在所研究的所有分离株中，K13基因的野生型表明该药物方案在研究区域仍然有效，且没有出现对青蒿素耐药的迹象。
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