Okui Masayuki, Goto Taichiro, Asakura Keisuke, Kamiyama Ikuo, Ohtsuka Takashi
Division of General Thoracic Surgery, Department of Surgery, School of Medicine, Keio University, Shinjuku-ku, Tokyo, 160-8582 Japan.
Springerplus. 2015 Dec 12;4:770. doi: 10.1186/s40064-015-1525-2. eCollection 2015.
No uniform consensus has been established regarding post-pneumonectomy lung regeneration. This study was undertaken to determine whether airway-instilled lung- or bone marrow-derived cells are able to differentiate and reconstitute as lung component cells in the course of post-pneumonectomy lung growth. Bone marrow cells or lung cells obtained from C57 black (BL)/6-GFP mice were intratracheally instilled into C57BL/6 mice treated with left pneumonectomy and cell differentiation was examined. It is unclear whether intratracheally instilled lung or bone marrow cells differentiate into non-hematopoietic cells after pneumonectomy. However, regardless of whether pneumonectomy is performed, intratracheally instilled bone marrow cells display a surface antigen profile that is similar to alveolar macrophages. Furthermore, these newly differentiated macrophages function similarly to resident macrophages in terms of TNF-α production, suggesting that bone marrow stem cells acquire the same macrophage phenotype. In conclusion, intratracheally instilled bone marrow cells adapt to the surrounding microenvironment, directly differentiating into alveolar macrophages, and remain in the alveolar space for at least 3 months.
关于肺切除术后肺再生，尚未达成统一共识。本研究旨在确定经气道注入的肺或骨髓来源的细胞在肺切除术后肺生长过程中是否能够分化并重构为肺组成细胞。从C57黑（BL）/6-GFP小鼠获得的骨髓细胞或肺细胞经气管内注入接受左肺切除的C57BL/6小鼠，并检查细胞分化情况。尚不清楚经气管内注入的肺或骨髓细胞在肺切除术后是否会分化为非造血细胞。然而，无论是否进行肺切除术，经气管内注入的骨髓细胞都表现出与肺泡巨噬细胞相似的表面抗原谱。此外，这些新分化的巨噬细胞在产生肿瘤坏死因子-α方面的功能与驻留巨噬细胞相似，这表明骨髓干细胞获得了相同的巨噬细胞表型。总之，经气管内注入的骨髓细胞适应周围微环境，直接分化为肺泡巨噬细胞，并在肺泡空间中至少停留3个月。
