Chiu Ping-Hsien, Weng Chen-Hsun, Yang Ruey-Jen
Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.
Medical Device Innovation Center, National Cheng Kung University, Tainan 70101, Taiwan.
Sensors (Basel). 2015 Dec 5;15(12):30704-15. doi: 10.3390/s151229824.
A fluidic microchip incorporating a convergent microchannel and a Nafion-nanoporous membrane is proposed for the preconcentration and separation of multi-species samples on a single platform. In the device, sample preconcentration is achieved by means of the ion concentration polarization effect induced at the micro/nano interface under the application of an external electric field, while species separation is achieved by exploiting the different electrophoretic mobilities of the sample components. The experimental results show that the device is capable of detecting C-reactive protein (CRP) with an initial concentration as low as 9.50 × 10(-6) mg/L given a sufficient preconcentration time and driving voltage. In addition, it is shown that a mixed-species sample consisting of three negatively-charged components (bovine serum albumin (BSA), tetramethylrhodamine(TAMRA) isothiocyanate-Dextran and fluorescent polymer beads) can be separated and preconcentrated within 20 min given a driving voltage of 100 V across 1 cm microchannel in length. In general, the present results confirm the feasibility of the device for the immunoassay or detection of various multi-species samples under low concentration in the biochemical and biomedical fields. The novel device can therefore improve the detection limit of traditional medical facilities.
本文提出了一种集成了收敛微通道和纳滤纳米多孔膜的流体微芯片，用于在单一平台上对多物种样品进行预浓缩和分离。在该装置中，样品预浓缩是通过在外部电场作用下，在微/纳界面处诱导产生的离子浓度极化效应来实现的，而物种分离则是通过利用样品组分不同的电泳迁移率来实现的。实验结果表明，在有足够的预浓缩时间和驱动电压的情况下，该装置能够检测初始浓度低至9.50×10⁻⁶mg/L的C反应蛋白（CRP）。此外，结果表明，在长度为1cm的微通道上施加100V的驱动电压时，由三种带负电荷的组分（牛血清白蛋白（BSA）、异硫氰酸四甲基罗丹明（TAMRA）-葡聚糖和荧光聚合物珠）组成的混合物种样品可在20分钟内实现分离和预浓缩。总体而言，目前的结果证实了该装置在生化和生物医学领域用于免疫分析或检测各种低浓度多物种样品的可行性。因此，这种新型装置可以提高传统医疗设备的检测限。
