Mourzina Yulia, Kalyagin Dmitry, Steffen Alfred, Offenhäusser Andreas
Institute of Thin Films and Interfaces, Center of Nanoelectronic Systems for Information Technology, Research Center Jülich, 52425 Jülich, Germany.
Talanta. 2006 Oct 15;70(3):489-98. doi: 10.1016/j.talanta.2005.12.063. Epub 2006 Feb 21.
In the present work, on-chip capillary electrophoresis for the separation of neuromediators is demonstrated. The influence of separation buffer (composition, pH, SDS additive), on-chip electrokinetic sample stacking, and surface pretreatment of the PDMS-PDMS and hybrid PDMS-glass devices on the electrokinetic characteristics of microfluidics (nu(eo), mu(eo), zeta) and separation performance of on-chip capillary electrophoresis of neuromediators have been investigated. It is demonstrated that for the effective separation of neuropeptides on elastomer-based microfluidic devices, on-chip sample stacking is necessary. Field-amplified sample stacking for electroosmotic flow supported on-chip separations of neuromediators and without special design of the sample injection scheme has been demonstrated. Electrophoretic separations of fluorescently labeled analytes have been achieved within tens of seconds at injection volumes of about 110pL, with plate numbers varying from <1000 to approximately 22,000. These results demonstrate that on-chip separation methods with hybrid PDMS-glass devices are perspective for the analysis of (neuro)peptides in small volumes.
在本工作中，展示了用于神经递质分离的芯片上毛细管电泳。研究了分离缓冲液（组成、pH值、SDS添加剂）、芯片上电动样品堆积以及PDMS-PDMS和混合PDMS-玻璃器件的表面预处理对微流体电动特性（电渗迁移率、电泳迁移率、zeta电位）和神经递质芯片上毛细管电泳分离性能的影响。结果表明，对于在基于弹性体的微流体器件上有效分离神经肽，芯片上样品堆积是必要的。已证明在没有特殊样品进样方案设计的情况下，用于电渗流的场放大样品堆积支持神经递质的芯片上分离。在进样体积约为110pL时，在数十秒内实现了荧光标记分析物的电泳分离，塔板数从<1000变化到约22000。这些结果表明，使用混合PDMS-玻璃器件的芯片上分离方法对于小体积（神经）肽的分析具有前景。
