Abad Sergi, Turon Xavier
Bioengineering Department, IQS, Ramon Llull University, Via Augusta 390, Barcelona 08017, Spain.
Mar Drugs. 2015 Dec 5;13(12):7275-84. doi: 10.3390/md13127064.
Aurantiochytrium limacinum, a marine heterotrophic protist/microalga has shown interesting yields of docosahexaenoic acid (DHA) when cultured with different carbon sources: glucose, pure and crude glycerol. A complete study in a lab-scale fermenter allowed for the characterization and comparison of the growth kinetic parameters corresponding to each carbon source. Artificial Marine Medium (AMM) with glucose, pure and crude glycerol offered similar biomass yields. The net growth rates (0.10-0.12 h(-1)), biomass (0.7-0.8 g cells/g Substrate) and product (0.14-0.15 g DHA/g cells) yields, as well as DHA productivity were similar using the three carbon sources. Viable potential applications to valorize crude glycerol are envisioned to avoid an environmental problem due to the excess of byproduct.
金藻门的破囊壶菌是一种海洋异养原生生物/微藻，当使用不同碳源（葡萄糖、纯甘油和粗甘油）进行培养时，它能产生可观的二十二碳六烯酸（DHA）产量。在实验室规模的发酵罐中进行的全面研究，对每种碳源对应的生长动力学参数进行了表征和比较。添加葡萄糖、纯甘油和粗甘油的人工海水培养基（AMM）产生的生物量产量相似。使用这三种碳源时，净生长速率（0.10 - 0.12 h⁻¹）、生物量（0.7 - 0.8 g细胞/g底物）和产物（0.14 - 0.15 g DHA/g细胞）产量以及DHA生产力都相似。为避免副产物过量造成的环境问题，设想了利用粗甘油的可行潜在应用。
