School of Bioengineering, FH Campus Wien - University of Applied Sciences, Muthgasse 62, 1190 Vienna, Austria; Department of Biotechnology, BOKU - VIBT University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria.
Department of Biotechnology, BOKU - VIBT University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria.
Bioresour Technol. 2014;152:499-504. doi: 10.1016/j.biortech.2013.11.041. Epub 2013 Nov 27.
Lactobacillus diolivorans was evaluated as a potential organism for production of 1,3-propanediol under industrially relevant conditions. Crude glycerol of different origins has been tested and showed no inhibitory effects on growth or production. Using crude glycerol from biodiesel production from palm oil 85 g/l 1,3-propanediol have been obtained with a productivity of 0.45 g/lh in a fed-batch cultivation. Sugar necessary for the formation of biomass was replaced with a hydrolysate from lignocellulosic material resulting in 75 g/l 1,3-propanediol and a productivity of 0.36 g/lh. Lignocellulosic hydrolysate contained the potential inhibitors furfural and 5-hydroxymethylfurfural at concentrations of 0.7 and 0.3 g/l, respectively. Addition of furfural and 5-hydroxymethylfurfural to batch cultures in said concentrations did not show inhibitory effects on growth or 1,3-propanediol production.
植物乳杆菌被评估为在工业相关条件下生产 1,3-丙二醇的潜在生物。已经测试了不同来源的粗甘油，发现其对生长或生产没有抑制作用。使用来自棕榈油生物柴油生产的粗甘油，在分批补料培养中获得了 85g/L 的 1,3-丙二醇，产率为 0.45g/lh。用于形成生物质的糖被木质纤维素材料的水解物所取代，产生了 75g/L 的 1,3-丙二醇和 0.36g/lh 的产率。木质纤维素水解物中分别含有 0.7 和 0.3g/L 的糠醛和 5-羟甲基糠醛这两种潜在抑制剂。在上述浓度下，向分批培养物中添加糠醛和 5-羟甲基糠醛，对生长或 1,3-丙二醇生产没有抑制作用。
