鸟苷酸环化酶的高压纸电泳测定法
High-voltage paper electrophoretic assay for guanyl cyclase.
作者信息
Mukerjee H
出版信息
J Chromatogr. 1978 Jun 1;153(1):247-52. doi: 10.1016/s0021-9673(00)89879-9.
PMID:26692
Abstract
A simple, sensitive and rapid technique is described, permitting separation of cGMP from GMP, GDP and GTP by the use of unidirectional high-voltage paper electrophoresis. The recovery of labeled cGMP in the assay of guanyl cyclase, by this procedure is 85-90%; the blank values (no enzyme) are negligible.
摘要
本文描述了一种简单、灵敏且快速的技术，该技术通过单向高压纸电泳法可将环磷酸鸟苷（cGMP）与鸟苷一磷酸（GMP）、鸟苷二磷酸（GDP）和鸟苷三磷酸（GTP）分离。采用此方法在鸟苷酸环化酶测定中标记的cGMP回收率为85% - 90%；空白值（无酶）可忽略不计。
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