一种测量乳腺组织中鸟苷酸环化酶活性的快速方法。

A rapid method for measuring guanylate cyclase activity in mammary tissue.

作者信息

Smith R D, Rillema J A

出版信息

Proc Soc Exp Biol Med. 1975 Dec;150(3):759-62. doi: 10.3181/00379727-150-39120.

Abstract

A simple and rapid method for measuring guanylate cyclase activity in broken cell preparations of biological tissues is described. This method employs the rate of conversion of [32P]GTP to [32P]cyclic-GMP. The product of this reaction is isolated by ion-exchange chromatography and by a ZnSO4-Ba(OH)2 precipitation at pH 5.7. Using this method, about 30-50 samples can be assayed for guanylate cyclase activity during a 5-6 hr period. The characteristics of this enzyme in the mammary gland were found to be similar to those described for other tissues using different methods for measuring guanylate cyclase activity.

摘要

本文描述了一种用于测量生物组织破碎细胞制剂中鸟苷酸环化酶活性的简单快速方法。该方法利用[32P]GTP转化为[32P]环磷酸鸟苷的速率。此反应产物通过离子交换色谱法以及在pH 5.7下的硫酸锌-氢氧化钡沉淀法进行分离。使用该方法，在5至6小时内可检测约30 - 50个样品的鸟苷酸环化酶活性。发现该酶在乳腺中的特性与使用其他测量鸟苷酸环化酶活性方法所描述的其他组织的特性相似。