马来西亚白纹伊蚊γ-氨基丁酸（GABA）受体A302S突变的首次分子基因分型。

First molecular genotyping of A302S mutation in the gamma aminobutyric acid (GABA) receptor in Aedes albopictus from Malaysia.

作者信息

Low V L, Vinnie-Siow W Y, Lim Y A L, Tan T K, Leong C S, Chen C D, Azidah A A, Sofian-Azirun M

机构信息

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

出版信息

Trop Biomed. 2015 Sep;32(3):554-6.

PMID:26695218

Abstract

Given the lack of molecular evidence in altered target-site insecticide resistance mechanism in Aedes albopictus (Skuse) worldwide, the present study aims to detect the presence of A302S mutation in the gene encoding the gamma aminobutyric acid receptor resistant to dieldrin (Rdl) in Ae. albopictus for the first time from its native range of South East Asia, namely Malaysia. World Health Organization (WHO) adult susceptibility bioassay indicated a relatively low level of dieldrin resistance (two-fold) in Ae. albopictus from Petaling Jaya, Selangor. However, PCR-RFLP and direct sequencing methods revealed the presence of the A302S mutation with the predomination of heterozygous genotype (40 out of 82 individuals), followed by the resistant genotype with 11 individuals. This study represents the first field evolved instance of A302S mutation in Malaysian insect species.

摘要

鉴于全球范围内白纹伊蚊（Skuse）改变靶标位点的杀虫剂抗性机制缺乏分子证据，本研究旨在首次从其东南亚原生地马来西亚检测白纹伊蚊中对狄氏剂抗性的γ-氨基丁酸受体编码基因中A302S突变的存在情况。世界卫生组织（WHO）的成蚊敏感性生物测定表明，来自雪兰莪八打灵再也的白纹伊蚊对狄氏剂的抗性水平相对较低（两倍）。然而，PCR-RFLP和直接测序方法揭示了A302S突变的存在，其中杂合基因型占主导（82个个体中有40个），其次是抗性基因型，有11个个体。本研究代表了马来西亚昆虫物种中A302S突变在野外进化的首个实例。