Torres-Pérez Mario, Tellez-Ballesteros Ruth Ivonne, Ortiz-López Leonardo, Ichwan Muhammad, Vega-Rivera Nelly Maritza, Castro-García Mario, Gómez-Sánchez Ariadna, Kempermann Gerd, Ramirez-Rodriguez Gerardo Bernabe
Laboratory of Neurogenesis, Division of Clinical Investigations, National Institute of Psychiatry "Ramón de la Fuente Muñiz", Calz. México-Xochimilco 101, 14370, México, D.F., México.
CRTD - Center for Regenerative Therapies Dresden, Tatzberg 47-79, 01307, Dresden, Germany.
PLoS One. 2015 Dec 22;10(12):e0145687. doi: 10.1371/journal.pone.0145687. eCollection 2015.
Resveratrol (RVTL) is a flavonoid found in red wine and has been publicized heavily as an anti-aging compound. Indeed, basic research confirms that although there is much hype in the promotion of RVTL, flavonoids such as RVTL have a wide range of biological effects. We here investigated the effects of RVTL treatment on hippocampal plasticity and memory performance in female Balb/C mice, a strain with low baseline levels of adult neurogenesis. Two weeks of treatment with RVTL (40 mg/kg) induced the production of new neurons in vivo by increasing cell survival and possibly precursor cell proliferation. In addition, RVTL decreased the number of apoptotic cells. The number of doublecortin (DCX)-expressing intermediate cells was increased. RVTL stimulated neuronal differentiation in vitro without effects on proliferation. In the dentate gyrus, RVTL promoted the formation and maturation of spines on granule cell dendrites. RVTL also improved performance in the step down passive avoidance test. The RVTL-treated mice showed increase in the levels of two key signaling proteins, phospho-Akt and phospho-PKC, suggesting the involvement of these signaling pathways. Our results support the vision that flavonoids such as resveratrol deserve further examination as plasticity-inducing compounds in the context of successful cognitive aging.
白藜芦醇(RVTL)是一种存在于红酒中的类黄酮,作为一种抗衰老化合物已被大量宣传。事实上,基础研究证实,尽管在推广RVTL方面有很多炒作,但像RVTL这样的类黄酮具有广泛的生物学效应。我们在此研究了RVTL处理对成年神经发生基线水平较低的雌性Balb/C小鼠海马可塑性和记忆表现的影响。用RVTL(40毫克/千克)处理两周可通过提高细胞存活率并可能促进前体细胞增殖来诱导体内新神经元的产生。此外,RVTL减少了凋亡细胞的数量。表达双皮质素(DCX)的中间细胞数量增加。RVTL在体外刺激神经元分化但对增殖无影响。在齿状回中,RVTL促进颗粒细胞树突棘的形成和成熟。RVTL还改善了被动回避试验中的表现。经RVTL处理的小鼠显示两种关键信号蛋白磷酸化Akt和磷酸化PKC的水平升高,表明这些信号通路参与其中。我们的结果支持这样一种观点,即在成功的认知衰老背景下,像白藜芦醇这样的类黄酮作为诱导可塑性的化合物值得进一步研究。