Ashida Yuto, Ueda Masahito
Opt Lett. 2016 Jan 1;41(1):72-5. doi: 10.1364/OL.41.000072.
We present a method that can simultaneously locate positions of overlapped multi-emitters at the theoretical-limit precision. We derive a set of simple equations whose solutions give the maximum likelihood estimator of multi-emitter positions. We compare the performance of our simultaneous localization analysis with the conventional single-molecule analysis for simulated images and show that our method can improve the time-resolution of super-resolution microscopy by an order of magnitude. In particular, we derive the information-theoretic bound on time resolution of localization-based super-resolution microscopy and demonstrate that the bound can be asymptotically attained by our analysis.
我们提出了一种能够以理论极限精度同时定位重叠多发射器位置的方法。我们推导了一组简单方程，其解给出了多发射器位置的最大似然估计值。我们将同时定位分析的性能与针对模拟图像的传统单分子分析进行了比较，结果表明我们的方法可以将超分辨率显微镜的时间分辨率提高一个数量级。特别是，我们推导了基于定位的超分辨率显微镜时间分辨率的信息论界限，并证明我们的分析可以渐近地达到该界限。
