Degrelle Séverine A
INRA, UMR1198 Biologie du Développement et Reproduction, F-78352 Jouy-en-Josas, France.
Genom Data. 2015 Aug 29;6:110-1. doi: 10.1016/j.gdata.2015.08.019. eCollection 2015 Dec.
The dataset described in this article pertains to the article by Hue et al. (2015) entitled "Primary bovine extra-embryonic cultured cells: A new resource for the study of in vivo peri-implanting phenotypes and mesoderm formation" [1]. In mammals, extra-embryonic tissues are essential to support not only embryo patterning but also embryo survival, especially in late implanting species. These tissues are composed of three cell types: trophoblast (bTCs), endoderm (bXECs) and mesoderm (bXMCs). Until now, it is unclear how these cells interact. In this study, we have established primary cell cultures of extra-embryonic tissues from bovine embryos collected at day-18 after artificial insemination. We used our homemade bovine 10K array (GPL7417) to analyze the gene expression profiles of these primary extra-embryonic cultured cells compared to the corresponding cells from in vivo micro-dissected embryos. Here, we described the experimental design, the isolation of bovine extra-embryonic cell types as well as the microarray expression analysis. The dataset has been deposited in Gene Expression Omnibus (GEO) (accession number GSE52967). Finally, these primary cell cultures were a powerful tool to start studying their cellular properties, and will further allow in vitro studies on cellular interactions among extra-embryonic tissues, and potentially between extra-embryonic vs embryonic tissues.
本文所述的数据集与Hue等人（2015年）发表的题为《原代牛胚外培养细胞：研究体内着床期表型和中胚层形成的新资源》[1]的文章相关。在哺乳动物中，胚外组织不仅对胚胎模式形成至关重要，而且对胚胎存活也至关重要，尤其是在着床较晚的物种中。这些组织由三种细胞类型组成：滋养层（bTCs）、内胚层（bXECs）和中胚层（bXMCs）。到目前为止，尚不清楚这些细胞是如何相互作用的。在本研究中，我们建立了从人工授精后第18天收集的牛胚胎中分离的胚外组织的原代细胞培养物。我们使用自制的牛10K芯片（GPL7417）分析这些原代胚外培养细胞与体内显微切割胚胎中相应细胞的基因表达谱。在此，我们描述了实验设计、牛胚外细胞类型的分离以及芯片表达分析。该数据集已存入基因表达综合数据库（GEO）（登录号GSE52967）。最后，这些原代细胞培养物是开始研究其细胞特性的有力工具，并将进一步允许对胚外组织之间以及潜在的胚外与胚胎组织之间的细胞相互作用进行体外研究。
