Del Rio Beatriz, Linares Daniel M, Redruello Begoña, Martin Maria Cruz, Fernandez Maria, de Jong Anne, Kuipers Oscar P, Ladero Victor, Alvarez Miguel A
Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands.
Genom Data. 2015 Oct 9;6:228-30. doi: 10.1016/j.gdata.2015.10.002. eCollection 2015 Dec.
Lactococcus lactis subsp. cremoris CECT 8666 (formerly GE2-14) is a dairy strain that catabolizes agmatine (a decarboxylated derivative of arginine) into the biogenic amine putrescine by the agmatine deiminase (AGDI) pathway [1]. The AGDI cluster of L. lactis is composed by five genes aguR, aguB, aguD, aguA and aguC. The last four genes are responsible for the deamination of agmatine to putrescine and are co-transcribed as a single policistronic mRNA forming the catabolic operon aguBDAC[1]. aguR encodes a transmembrane protein that functions as a one-component signal transduction system that senses the agmatine concentration of the medium and accordingly regulates the transcription of aguBDAC[2], which is also transcriptionally regulated by carbon catabolic repression (CCR) via glucose, but not by other sugars such as lactose and galactose [1], [3]. Here we report the transcriptional profiling of the aguR gene deletion mutant (L. lactis subsp. cremoris CECT 8666 ∆aguR) [2] compared to the wild type strain, both grown in M17 medium with galactose as carbon source and supplemented with agmatine. The transcriptional profiling data of AguR-regulated genes were deposited in the Gene Expression Omnibus (GEO) database under accession no. GSE59514.
乳酸乳球菌乳脂亚种CECT 8666（原名GE2 - 14）是一种乳源菌株，它通过胍丁胺脱亚氨酶（AGDI）途径将胍丁胺（精氨酸的一种脱羧衍生物）分解代谢为生物胺腐胺[1]。乳酸乳球菌的AGDI基因簇由五个基因aguR、aguB、aguD、aguA和aguC组成。后四个基因负责将胍丁胺脱氨生成腐胺，并作为一个单一的多顺反子mRNA共同转录，形成分解代谢操纵子aguBDAC[1]。aguR编码一种跨膜蛋白，其作为单组分信号转导系统发挥作用，可感知培养基中胍丁胺的浓度，并相应地调节aguBDAC的转录[2]，aguBDAC的转录也受到碳分解代谢阻遏（CCR）通过葡萄糖的调控，但不受乳糖和半乳糖等其他糖类的调控[1,3]。在此，我们报告了与野生型菌株相比，胍丁胺脱亚氨酶基因缺失突变体（乳酸乳球菌乳脂亚种CECT 8666 ∆aguR）[2]的转录谱分析，二者均在以半乳糖为碳源并添加胍丁胺的M17培养基中生长。AguR调控基因的转录谱分析数据已存入基因表达综合数据库（GEO），登录号为GSE59514。
