Hsieh J C, Liu L F, Chen W L, Tam M F
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
Biochem Biophys Res Commun. 1989 Aug 15;162(3):1147-54. doi: 10.1016/0006-291x(89)90793-6.
A full-length cDNA clone was isolated for rat liver Yb1 glutathione S-transferase (EC 2.5.1.18). The coding sequence of Yb1 cDNA was inserted into a baculovirus vector for infection of Spodoptera frugiperda (SF9) cells. The enzymatically active recombinant Yb1 glutathione S-transferase protein has a native molecular weight of 42,000 daltons (by molecular sieve chromatography), a subunit molecular weight of 26,500 daltons (by SDS-polyacrylamide gel electrophoresis), a pI of 8.4 and an extinction coefficient E1%280 of 5.6 +/- 0.4.
分离得到大鼠肝脏Yb1谷胱甘肽S-转移酶（EC 2.5.1.18）的全长cDNA克隆。将Yb1 cDNA的编码序列插入杆状病毒载体，用于感染草地贪夜蛾（SF9）细胞。具有酶活性的重组Yb1谷胱甘肽S-转移酶蛋白的天然分子量为42,000道尔顿（通过分子筛色谱法），亚基分子量为26,500道尔顿（通过SDS-聚丙烯酰胺凝胶电泳），pI为8.4，消光系数E1%280为5.6±0.4。
