邻香草醛（一种有效的共诱变剂）对适应性反应诱导的特异性抑制作用。

A specific inhibition of induction of adaptive response by o-vanillin, a potent comutagen.

作者信息

Takahashi K, Sekiguchi M, Kawazoe Y

机构信息

Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.

出版信息

Biochem Biophys Res Commun. 1989 Aug 15;162(3):1376-81. doi: 10.1016/0006-291x(89)90826-7.

DOI:10.1016/0006-291x(89)90826-7

PMID:2669748

Abstract

The present study revealed that potent comutagenic activity of o-vanillin in mutagenesis by N-methyl-N-nitrosourea (MNU) is a consequence of inhibition of the transcriptional promoting capacity of methylated O6-methylguanine-DNA methyltransferase (MGTase). As evidence of this, in the presence of o-vanillin, there were (i) dose-dependent decreases in MGTase induced in E. coli exposed to MNU, (ii) inhibition of ada gene promotion determined in the ada'-lacZ' assay system, and (iii) minimal inhibition of the methyl transfer capacity of MGTase in a subcellular system.

摘要

本研究表明，邻香草醛在N-甲基-N-亚硝基脲（MNU）诱变中具有强大的共诱变活性，这是抑制甲基化的O6-甲基鸟嘌呤-DNA甲基转移酶（MGTase）转录促进能力的结果。作为对此的证据，在邻香草醛存在的情况下，有以下情况：（i）暴露于MNU的大肠杆菌中诱导的MGTase呈剂量依赖性降低；（ii）在ada'-lacZ'检测系统中测定的ada基因促进受到抑制；（iii）在亚细胞系统中MGTase的甲基转移能力受到最小程度的抑制。