光功能化对大鼠股骨节段性骨缺损处Ti6Al4V螺钉稳定性的影响。
Effect of Photofunctionalization on Ti6Al4V Screw Stability Placed in Segmental Bone Defects in Rat Femurs.
作者信息
Hirota Makoto, Tanaka Miyuki, Ishijima Manabu, Iwasaki Chika, Park Wonhee, Ogawa Takahiro
机构信息
Associate Professor, Laboratory for Bone and Implant Sciences, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, University of California, Los Angeles, School of Dentistry, Los Angeles, CA; and Visiting Scholar, Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Visiting Scholar, Laboratory for Bone and Implant Sciences, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, University of California, Los Angeles, School of Dentistry, Los Angeles, CA.
出版信息
J Oral Maxillofac Surg. 2016 Apr;74(4):861.e1-16. doi: 10.1016/j.joms.2015.11.016. Epub 2015 Nov 23.
PURPOSE
Ultraviolet-mediated photofunctionalization is a new technology to improve bone and titanium integration. We hypothesized that photofunctionalization would enhance the stability of titanium screws used for segmental bone defects.
MATERIALS AND METHODS
Disks were prepared of a titanium alloy (Ti6Al4V) for an in vitro study to evaluate the attachment, proliferation, and differentiation of osteoblasts. Commercially available Ti6Al4V screws were used in vivo. Segmental bone defects were created in rat femurs as an immediate loading reconstruction model. The defects were reconstructed with commercially available titanium plates and Ti6Al4V screws, with or without photofunctionalization. The screw survival rates and mechanical stability were evaluated at 2 and 4 weeks, and the bone formation around the screws was analyzed.
RESULTS
Osteoblasts showed greater attachment, proliferation, and differentiation on the photofunctionalized Ti6Al4V disks. Photofunctionalized screws had significantly greater survival rates and mechanical stability at 2 and 4 weeks. The bone formation around the photofunctionalized screws was significantly greater than that around the untreated screws at 4 weeks.
CONCLUSIONS
The results of the present study have demonstrated the efficacy of photofunctionalization on enhancing the survival and stability of Ti6Al4V screws under a loaded condition in the reconstruction of segmental defects. This was associated with increased bioactivity and bone formation around the photofunctionalized Ti6Al4V material.
目的
紫外线介导的光功能化是一种改善骨与钛结合的新技术。我们假设光功能化会增强用于节段性骨缺损的钛螺钉的稳定性。
材料与方法
制备钛合金（Ti6Al4V）圆盘用于体外研究，以评估成骨细胞的附着、增殖和分化。市售的Ti6Al4V螺钉用于体内实验。在大鼠股骨中制造节段性骨缺损作为即刻加载重建模型。缺损用市售钛板和Ti6Al4V螺钉重建，分为光功能化组和非光功能化组。在2周和4周时评估螺钉存活率和力学稳定性，并分析螺钉周围的骨形成情况。
结果
成骨细胞在光功能化的Ti6Al4V圆盘上表现出更强的附着、增殖和分化能力。光功能化螺钉在2周和4周时具有显著更高的存活率和力学稳定性。在4周时，光功能化螺钉周围的骨形成明显多于未处理螺钉周围的骨形成。
结论
本研究结果证明了光功能化在节段性缺损重建中加载条件下增强Ti6Al4V螺钉存活率和稳定性方面的有效性。这与光功能化Ti6Al4V材料周围生物活性和骨形成增加有关。
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