Huo Zhen, Zeng Xuan, Wu Shafei, Wu Huanwen, Meng Yunxiao, Liu Yuanyuan, Luo Yufeng, Cao Jinling, Liang Zhiyong
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China; E-mail:
Zhonghua Bing Li Xue Za Zhi. 2015 Aug;44(8):582-6.
To explore the diagnostic value of MYB protein expression for adenoid cystic carcinoma and its differential diagnosis from other salivary gland tumors, and to further investigate the status of MYB gene copy number.
MYB expression was studied by immunohistochemistry in 34 adenoid cystic carcinomas, 55 non-adenoid cystic carcinomas (other salivary gland tumors) including 10 pleomorphic adenomas, 10 basal cell adenomas, 10 epithelial-myoepithelial carcinomas, 9 basal cell adenocarcinomas, 8 mucoepidermoid carcinomas, 4 carcinoma in pleomorphic adenomas, and 4 polymorphous low-grade adenocarcinoma. MYB gene copy number status was detected by FISH in MYB protein-positive cases.
82.4% (28/34) of adenoid cystic carcinomas were MYB protein-positive, compared with 9.1% (5/55) of non-adenoid cystic carcinomas, and the difference between the two groups was statistically significant (P < 0.01). 2/18 of adenoid cystic carcinomas had duplication of MYB gene by FISH, and all non-adenoid cystic carcinomas were negative although the difference was not statistically significant (P = 0.435).
MYB protein expression is a useful diagnostic marker for adenoid cystic carcinomas in its separation from other salivary gland tumors. In addition, duplication of MYB gene is no a major mechanism for the MYB protein overexpression.
探讨MYB蛋白表达对腺样囊性癌的诊断价值及其与其他涎腺肿瘤的鉴别诊断，并进一步研究MYB基因拷贝数状态。
采用免疫组织化学法检测34例腺样囊性癌、55例非腺样囊性癌（其他涎腺肿瘤）中MYB的表达，其中非腺样囊性癌包括10例多形性腺瘤、10例基底细胞腺瘤、10例上皮-肌上皮癌、9例基底细胞腺癌、8例黏液表皮样癌、4例多形性腺瘤中的癌和4例多形性低度腺癌。对MYB蛋白阳性病例采用荧光原位杂交（FISH）检测MYB基因拷贝数状态。
腺样囊性癌中82.4%（28/34）MYB蛋白阳性，非腺样囊性癌中为9.1%（5/55），两组差异有统计学意义（P<0.01）。FISH检测显示18例腺样囊性癌中有2例MYB基因扩增，所有非腺样囊性癌均为阴性，差异无统计学意义（P=0.435）。
MYB蛋白表达是腺样囊性癌与其他涎腺肿瘤鉴别的有用诊断标志物。此外，MYB基因扩增不是MYB蛋白过表达的主要机制。
