Zhang Xueming, Meng Lin, Liu Bo, Hu Yunyan, Cheng Feng, Liang Jianli, Aarts Mark G M, Wang Xiaowu, Wu Jian
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun Southern Street 12, 100081 Beijing, China.
Laboratory of Genetics, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, the Netherlands.
Plant Sci. 2015 Dec;241:211-20. doi: 10.1016/j.plantsci.2015.10.007. Epub 2015 Oct 23.
Long days and vernalization accelerate the transition from vegetative growth to reproductive growth in Brassica rapa. Bolting before plants reach the harvesting stage is a serious problem in B. rapa vegetable crop cultivation. The genetic dissection of flowering time is important for breeding of premature bolting-resistant B. rapa crops. Using a recombinant inbred line (RIL) population, we twice detected two major quantitative trait loci (QTLs) for flowering time in two different growing seasons that were located on chromosomes A02 and A07, respectively. We hypothesized that an orthologue of the Arabidopsis thaliana FLOWERING LOCUS T (FT) gene, named as BrFT2, was the candidate gene underlying the QTL localized to A07. A transposon insertion in the second intron of BrFT2 was detected in one of the parental lines, which was predicted to generate a loss-of-function allele. Transcription analysis revealed that the BrFT2 transcript was not present in the parental line that harbored the mutated allele. RILs carrying only the mutated BrFT2 allele showed delayed flowering regardless of growing seasons when compared to RILs carrying the wild-type BrFT2 allele. These data suggest that BrFT2 is involved in flowering time regulation in controlling flowering time in B. rapa.
长日照和春化作用加速了白菜从营养生长向生殖生长的转变。在白菜蔬菜作物栽培中，植株在达到收获阶段之前抽薹是一个严重问题。开花时间的遗传剖析对于培育抗早熟抽薹的白菜作物很重要。利用重组自交系（RIL）群体，我们在两个不同的生长季节两次检测到两个控制开花时间的主要数量性状位点（QTL），分别位于A02和A07染色体上。我们推测拟南芥开花位点T（FT）基因的一个直系同源基因，命名为BrFT2，是位于A07上的QTL的候选基因。在一个亲本系中检测到BrFT2第二个内含子中的转座子插入，预计会产生一个功能缺失等位基因。转录分析表明，携带突变等位基因的亲本系中不存在BrFT2转录本。与携带野生型BrFT2等位基因的RIL相比，仅携带突变型BrFT2等位基因的RIL无论生长季节如何，开花均延迟。这些数据表明，BrFT2参与了白菜开花时间的调控。
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