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构建用于低温表达系统以异源生产低温适应酶。

Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes.

作者信息

Bjerga Gro Elin Kjæreng, Lale Rahmi, Williamson Adele Kim

机构信息

a University of Tromsø, Norstruct, Department of Chemistry, Faculty of Science and Technology , Tromsø , Norway.

b Norwegian University of Science and Technology , Department of Biotechnology , Trondheim , Norway.

出版信息

Bioengineered. 2016;7(1):33-8. doi: 10.1080/21655979.2015.1128589. Epub 2015 Dec 28.

Abstract

Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased toward those that can be stably produced in commonly used mesophilic host systems. Two main strategies are currently being explored for the development of low-temperature expression in bacterial hosts: (i) low-temperature adaption of existing mesophilic expression systems, and (ii) development of new psychrophilic hosts. These developments include genetic engineering of the expression cassettes to optimize the promoter/operator systems that regulate heterologous expression. In this addendum we present our efforts in the development of such low-temperature expression systems, and speculate about future advancements in the field and potential applications.

摘要

在常用的嗜温表达系统中生产嗜冷酶,会受到重组酶在最佳宿主生长温度下固有稳定性低的阻碍。除非低温表达策略取得进展,否则嗜冷酶的研究最终可能会偏向于那些能够在常用嗜温宿主系统中稳定产生的酶。目前正在探索两种主要策略来开发细菌宿主中的低温表达:(i)对现有嗜温表达系统进行低温适应性改造,以及(ii)开发新的嗜冷宿主。这些进展包括对表达盒进行基因工程改造,以优化调节异源表达的启动子/操纵子系统。在本附录中,我们介绍了我们在开发此类低温表达系统方面所做的努力,并推测了该领域未来的进展和潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa67/4878266/727d317980bb/kbie-07-01-1128589-g001.jpg

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