Behera Bijay Kumar, Kumari Kavita, Baisvar Vishwamitra Singh, Rout Ajaya Kumar, Pakrashi Sudip, Paria Prasenjet, Jena J K
a Fish Biotechnology Laboratory, ICAR-Central Inland Fisheries Research Institute , Barrackpore , West Bengal , Kolkata , India , and.
b ICAR-National Bureau of Fish Genetics Resources , Lucknow , Uttar Pradesh , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Jan;28(1):7-8. doi: 10.3109/19401736.2015.1106517. Epub 2015 Dec 28.
In the present study, the complete mitochondrial genome sequence of Labeo gonius is reported using PGM sequencer (Ion Torrent). The complete mitogenome of L. gonius is obtained by the de novo sequences assembly of genomic reads using the Torrent Mapping Alignment Program (TMAP) which is 16 614 bp in length. The mitogenome of L. gonius comprised of 13 protein-coding genes, 22 tRNAs, 2 rRNA genes, and D-loop as control region along with gene order and organization, being similar to most of other fish mitogenomes of NCBI databases. The mitogenome in the present study has 99% similarity to the complete mitogenome sequence of Labeo fimbriatus, as reported earlier. The phylogenetic analysis of Cypriniformes depicted that their mitogenomes are closely related to each other. The complete mitogenome sequence of L. gonius would be helpful in understanding the population genetics, phylogenetics, and evolution of Indian Carps.
在本研究中，使用PGM测序仪（Ion Torrent）报道了似野结鱼的完整线粒体基因组序列。似野结鱼的完整线粒体基因组是通过使用Torrent Mapping Alignment Program（TMAP）对基因组读数进行从头序列组装获得的，长度为16614 bp。似野结鱼的线粒体基因组由13个蛋白质编码基因、22个tRNA、2个rRNA基因和作为控制区的D环组成，其基因顺序和组织与NCBI数据库中大多数其他鱼类线粒体基因组相似。如先前报道，本研究中的线粒体基因组与流苏结鱼的完整线粒体基因组序列有99%的相似性。鲤形目的系统发育分析表明，它们的线粒体基因组彼此密切相关。似野结鱼的完整线粒体基因组序列将有助于理解印度鲤科鱼类的群体遗传学、系统发育学和进化。
