Zhang Huajun, Peng Yun, Zhang Su, An Xinli, Li Yi, Zheng Wei, Zheng Tianling
Wei Sheng Wu Xue Bao. 2015 Jul 4;55(7):834-42.
A strain named BS01 showed strong algicidal activity to Alexandrium tamarense and we got algicidal compound (2-isobutoxyphenyl) amine from BS01 to study its algicidal effect on A. tamarense.
We studied the algicidal mechanism of (2-isobutoxyphenyl) amine on photosynthetic process, antioxidant enzyme activities and morphological change of A. tamarense.
After 24 hours treatment with (2-isobutoxyphenyl) amine, algicidal activity was 84. 1% with the concentration of 20 µg/mL. The compound could induce a reactive oxygen species burst in P. globosa in 0. 5 hours which could cause serious oxidative damage to algal cells. The Fv/Fm value which could reflect photosystem II (PS II) electron flow status also decreased. To eliminate the excess ROS, the activities of the antioxidant systems (including superoxide dismutase and catalase) increased significantly during exposure. Transmission electron microscope analysis showed obvious morphological modifications of chloroplast dismantling as a part of the algicidal process.
These results indicated that the lysis mechanism of algicidal compound on algae may primarily be the increasing level of ROS in the algal cells.
一株名为BS01的菌株对塔玛亚历山大藻显示出较强的杀藻活性，我们从BS01中获得了杀藻化合物（2-异丁氧基苯基）胺，以研究其对塔玛亚历山大藻的杀藻效果。
我们研究了（2-异丁氧基苯基）胺对塔玛亚历山大藻光合作用过程、抗氧化酶活性和形态变化的杀藻机制。
用（2-异丁氧基苯基）胺处理24小时后，浓度为20μg/mL时杀藻活性为84.1%。该化合物可在0.5小时内诱导球形棕囊藻产生活性氧爆发，这可能对藻类细胞造成严重的氧化损伤。反映光系统II（PS II）电子流动状态的Fv/Fm值也降低。为了消除过量的活性氧，暴露期间抗氧化系统（包括超氧化物歧化酶和过氧化氢酶）的活性显著增加。透射电子显微镜分析显示，作为杀藻过程的一部分，叶绿体解体有明显的形态改变。
这些结果表明，杀藻化合物对藻类的裂解机制可能主要是藻类细胞内活性氧水平的升高。
