Khan Hafiz Azhar Ali, Akram Waseem, Arshad Muhammad, Hafeez Faisal
Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
Department of Entomology, University of Agriculture, Faisalabad, Pakistan.
Parasitol Res. 2016 Apr;115(4):1385-90. doi: 10.1007/s00436-015-4872-6. Epub 2015 Dec 28.
The house fly, Musca domestica, is a serious pest of public health importance with the ability to develop insecticide resistance. The focus of the present study was to evaluate toxicity and resistance of the field collected house flies from Punjab, Pakistan, against insect growth regulator (IGR) insecticides. House flies collected from six different localities exhibited very low levels of resistance to cyromazine, triflumuron, and methoxyfenozide compared with the Lab-susceptible reference strain, with resistance ratios (RRs) ranging between 3.56- to 8.19-fold, 1.45- to 3.68-fold, and 2.20- to 8.60-fold, respectively. However, very low to low levels of resistance were observed for pyriproxyfen and very low to moderate levels for lufenuron with RRs ranged from 4.13- to 11.63-fold to 8.57- to 22.75-fold, respectively. There was a significant correlation between RRs of cyromazine and triflumuron (r = 0.976, p < 0.001), suggesting the possibility for cross-resistance. Susceptibility status of different IGRs in Pakistani strains of house flies is reported here for the first time. The trend observed in decreasing susceptibility towards different IGR insecticides tested will continue unless resistance management practices are followed.
家蝇（Musca domestica）是一种对公共卫生具有重要影响的严重害虫，具有产生杀虫剂抗性的能力。本研究的重点是评估从巴基斯坦旁遮普省采集的野外家蝇对昆虫生长调节剂（IGR）类杀虫剂的毒性和抗性。与实验室敏感参考品系相比，从六个不同地点采集的家蝇对环丙氨嗪、氟虫脲和甲氧虫酰肼的抗性水平非常低，抗性比率（RRs）分别在3.56至8.19倍、1.45至3.68倍和2.20至8.60倍之间。然而，观察到吡丙醚的抗性水平非常低至低，虱螨脲的抗性水平非常低至中等，RRs分别为4.13至11.63倍和8.57至22.75倍。环丙氨嗪和氟虫脲的RRs之间存在显著相关性（r = 0.976，p < 0.001），表明存在交叉抗性的可能性。本文首次报道了巴基斯坦家蝇品系中不同IGR的敏感性状况。除非采取抗性管理措施，否则所测试的不同IGR杀虫剂的敏感性下降趋势将持续下去。
