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三种免疫诊断试验在检测自然感染旋毛虫病大鼠中的比较。

Comparison of three immunodiagnostic tests in detection of trichinosis in naturally infected rats.

作者信息

Morsy T A, Makarem S S, Abou-el-Seoud S F, Ambroise-Thomas P

出版信息

J Egypt Soc Parasitol. 1989 Dec;19(2):465-9.

PMID:2671174
Abstract

The Counter current immunoelectrophoresis (CEP), the Enzyme linked immunosorbent assay (ELISA) and the Indirect immunofluorescent (IF) were evaluated in detection of antibodies in naturally infected rats. The test subjects included ten infected rats as indicated by digestion method of muscle biopsy and 13 rats free from trichinosis. Of the immunodiagnostic tests evaluated, the CEP gave the highest diagnostic accuracy. The ELISA was the least accurate. The high sensitivity and specificity of the CEP for detection of anti-trichinellal antibodies suggest that it could be developed as a serodiagnostic test for trichinosis.

摘要

对逆流免疫电泳(CEP)、酶联免疫吸附测定(ELISA)和间接免疫荧光法(IF)在检测自然感染大鼠抗体中的应用进行了评估。测试对象包括10只经肌肉活检消化法证实感染的大鼠以及13只未感染旋毛虫病的大鼠。在所评估的免疫诊断试验中,CEP的诊断准确性最高。ELISA的准确性最低。CEP检测抗旋毛虫抗体的高敏感性和特异性表明,它可开发成为旋毛虫病的血清学诊断试验。

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