Honma S, Endou M, Uchiyama M, Nakamura M, Kanazawa K, Takeuchi S
Department of Obstetrics and Gynecology, School of Medicine, Niigata University.
Nihon Sanka Fujinka Gakkai Zasshi. 1989 Jun;41(6):737-43.
The expression of MHC class II antigens (HLA-DR, -DP, -DQ) on trophoblast was analyzed by an immunoperoxidase technique using monoclonal antibodies. Tissues were obtained after pregnancy hysterectomy with the medical indications and after normal deliveries. They were snap-frozen in liquid nitrogen, cut in a cryostat and fixed in acetone for 10 minutes. Mouse monoclonal antibodies used in this study were anti-HLA-ABC (W6/32, Sera lab), anti-HLA-DR (Cappel), anti-HLA-DP (B7/21, Becton Dickinson) and anti-HLA-DQ (anti-Leu-10, Becton Dickinson) in addition to anti-cytokeratin (CAM5.2, Becton Dickinson) to recognize trophoblast. In the first trimester, HLA-DP could be identified on cytotrophoblastic cell column, cell island, extravillous trophoblast invading the decidua basalis, which were positive for HLA-ABC, and villous cytotrophoblast. In term placenta, villous syncytiotrophoblast and extra-villous trophoblast found in the decidua were positive for HLA-DP. In contrast, no trophoblast reacted with anti-HLA-DR or anti-HLA-DQ.
采用免疫过氧化物酶技术，使用单克隆抗体分析滋养层细胞上MHCⅡ类抗原（HLA - DR、- DP、- DQ）的表达。组织取自因医学指征行妊娠子宫切除术后以及正常分娩后。将组织在液氮中速冻，在低温恒温器中切片，然后用丙酮固定10分钟。本研究中使用的小鼠单克隆抗体包括抗HLA - ABC（W6/32，血清实验室）、抗HLA - DR（卡佩尔）、抗HLA - DP（B7/21，贝克顿·迪金森公司）和抗HLA - DQ（抗Leu - 10，贝克顿·迪金森公司），此外还有抗细胞角蛋白（CAM5.2，贝克顿·迪金森公司）用于识别滋养层细胞。在孕早期，HLA - DP可在细胞滋养层细胞柱、细胞岛、侵入基底蜕膜的绒毛外滋养层细胞（这些细胞HLA - ABC呈阳性）以及绒毛细胞滋养层细胞上被识别。在足月胎盘中，绒毛合体滋养层细胞以及在蜕膜中发现的绒毛外滋养层细胞HLA - DP呈阳性。相比之下，没有滋养层细胞与抗HLA - DR或抗HLA - DQ发生反应。
