Baranowska Irena, Hejniak Judyta, Magiera Sylwia
Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Gliwice, Poland.
Chirality. 2016 Feb;28(2):147-52. doi: 10.1002/chir.22559. Epub 2015 Dec 29.
A rapid and effective RP-UHPLC-DAD method for enantioseparation of three flavanones, i.e., flavanone, naringenin, and hesperetin, was developed and validated. Chromatographic separation of the analytes was performed using a Chiralpak AD-3R analytical column under reverse phase conditions with methanol as the mobile phase. The method was validated in the concentration range of 0.2 to 50 µg/mL for enantiomers of flavanone and 0.5 to 50 µg/mL for enantiomers of naringenin and hesperetin. The limits of quantification were between 0.03 to 0.5 µg/mL. Intraday and interday precision were below 14% and accuracy varied from 0.04 to 8.17%.
建立并验证了一种快速有效的反相超高效液相色谱-二极管阵列检测法（RP-UHPLC-DAD），用于分离三种黄烷酮对映体，即黄烷酮、柚皮素和橙皮素。在反相条件下，使用Chiralpak AD-3R分析柱，以甲醇为流动相进行分析物的色谱分离。该方法对黄烷酮对映体的验证浓度范围为0.2至50μg/mL，对柚皮素和橙皮素对映体的验证浓度范围为0.5至50μg/mL。定量限在0.03至0.5μg/mL之间。日内和日间精密度均低于14%，准确度在0.04%至8.17%之间。
