França Angela, Cerca Nuno
CEB - Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
CEB - Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
Pathog Dis. 2016 Mar;74(2). doi: 10.1093/femspd/ftv125. Epub 2015 Dec 27.
Staphylococcus epidermidis is frequently associated with the emergence of medical-device-associated bloodstream infections, due to its ability to form biofilms on the surface of vascular catheters. Although these biofilms may be in continuous contact with human blood, how S. epidermidis biofilm cells interact with blood and its cellular and soluble components is poorly understood. Herein, we evaluated biofilm structure, biofilm cells culturability and viability, and the transcription of a panel of genes associated with S. epidermidis biofilms virulence, upon interaction with whole human blood or plasma. Our results showed that although whole human blood caused significant alterations in biofilm structure and in the number of culturable and viable cells, plasma was the main regulator of the transcription of genes with central role in biofilm formation, maturation and immune evasion. These findings highlight the urgent need to intensify studies aiming to evaluate the impact of host soluble factors on S. epidermidis biofilms fitness and persistence.
表皮葡萄球菌经常与医疗设备相关的血流感染的出现有关，这是因为它能够在血管导管表面形成生物膜。尽管这些生物膜可能与人体血液持续接触，但表皮葡萄球菌生物膜细胞如何与血液及其细胞和可溶性成分相互作用却知之甚少。在此，我们评估了与全血或血浆相互作用后生物膜的结构、生物膜细胞的可培养性和活力，以及一组与表皮葡萄球菌生物膜毒力相关基因的转录情况。我们的结果表明，尽管全血会导致生物膜结构以及可培养和活细胞数量发生显著变化，但血浆是在生物膜形成、成熟和免疫逃避中起核心作用的基因转录的主要调节因子。这些发现凸显了迫切需要加强旨在评估宿主可溶性因子对表皮葡萄球菌生物膜适应性和持久性影响的研究。
