来自巴斯德毕赤酵母的乙醇氧化酶的结晶
Crystallization of alcohol oxidase from Pichia pastoris.
作者信息
Boys C W, Hill D J, Stockley P G, Woodward J R
机构信息
Astbury Department of Biophysics, University of Leeds, U.K.
出版信息
J Mol Biol. 1989 Jul 5;208(1):211-2. doi: 10.1016/0022-2836(89)90099-5.
Crystals of alcohol oxidase purified from Pichia pastoris were grown in microdialysis buttons in a solution of polyethylene glycol, sodium chloride and sodium azide. The crystals were stratified along the major axis and up to 3 mm in length. X-ray diffraction experiments indicated a space group of P2(1) and unit cell dimensions of a = 157.3 A, b = 171.5 A and c = 231.6 A. Crystals diffract to beyond 2.7 A and are suitable for X-ray structure analysis.
从巴斯德毕赤酵母中纯化得到的乙醇氧化酶晶体，在含有聚乙二醇、氯化钠和叠氮化钠的溶液中于微透析纽扣中生长。晶体沿长轴分层，长度可达3毫米。X射线衍射实验表明其空间群为P2(1)，晶胞参数为a = 157.3 Å，b = 171.5 Å，c = 231.6 Å。晶体的衍射分辨率超过2.7 Å，适合进行X射线结构分析。
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