Sharaf Radwa, Mempel Thorsten R, Murooka Thomas T
Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy andImmunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02129, USA.
Departments of Immunology and Medical Microbiology, University of Manitoba, 750 McDermot Ave, Rm 433, Winnipeg, MB, Canada, R3E 0T5.
Methods Mol Biol. 2016;1354:189-201. doi: 10.1007/978-1-4939-3046-3_13.
The introduction of multiphoton microscopy has dramatically broadened the scope of intravital imaging studies and has allowed researchers to validate and refine basic mechanistic concepts in many areas of biology within the context of physiologically relevant tissue microenvironments. This has also led to new insights into the behavior of immune cells at steady state, and how their behaviors are altered during an immune response. At the same time, advances in the humanized mouse model have allowed for in vivo studies of strictly human pathogens, such as HIV-1. Here, we describe in detail an intravital microscopy approach to visualize the dynamic behavior of HIV-infected T cells within the lymph nodes of live, anesthetized humanized mice.
多光子显微镜的引入极大地拓宽了活体成像研究的范围，使研究人员能够在生理相关的组织微环境中验证和完善生物学许多领域的基本机制概念。这也为稳态下免疫细胞的行为以及它们在免疫反应过程中的行为如何改变带来了新的见解。与此同时，人源化小鼠模型的进展使得对诸如HIV-1等严格的人类病原体进行体内研究成为可能。在这里，我们详细描述一种活体显微镜方法，以可视化感染HIV的T细胞在活的、麻醉的人源化小鼠淋巴结内的动态行为。
