Mempel Thorsten R
Center for Immunology and Inflammatory Diseases and Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA.
Methods Mol Biol. 2010;616:181-92. doi: 10.1007/978-1-60761-461-6_12.
Novel nonlinear optical imaging modalities, most prominently multiphoton microscopy, allow for the direct in vivo visualization of dynamic biological processes in experimental animals at high spatial and temporal resolution. This has led to important new insights into the orchestration of adaptive immune responses. Here we describe in detail a technique that allows for the monitoring of the cytotoxic function of CD8(+) effector T cells in tumor-draining lymph nodes of mice at the single-cell level in vivo using multiphoton intravital microscopy.
新型非线性光学成像模式，最突出的是多光子显微镜，能够在高空间和时间分辨率下对实验动物体内的动态生物学过程进行直接可视化。这为适应性免疫反应的调控带来了重要的新见解。在这里，我们详细描述一种技术，该技术使用多光子活体显微镜在体内单细胞水平监测小鼠肿瘤引流淋巴结中CD8(+)效应T细胞的细胞毒性功能。
