Wen Chorng-Kai, Lee Tzung-Yan
Graduate Institute of Clinical Medicine Sciences, College of Medicine, Chang Gung University, Kwei-Shan, Taiwan, R.O.C..
Graduate Institute of Traditional Chinese Medicine, College of Medicine, Chang Gung University, 259, Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, 333, Taiwan, R.O.C..
BMC Complement Altern Med. 2015 Dec 29;15:452. doi: 10.1186/s12906-015-0977-9.
Electroacupuncture (EA) shows anti-inflammation and several pleiotropic effects that interact with metabolic pathways. In the present study we tested the hypothesis that EA prevents inflammatory response and weight gain in obese mice through modulation of hypoxia-inducible factors-1α (HIF1-α)-dependent pathways in white adipose tissues.
Mice were divided in 4 groups: Non-obese, ob/ob, ob/ob submitted to 3 treatments, ob/ob submitted to 7 treatments. Low-frequency EA (2 Hz) was applied at the Zusanli (ST36) acupoint 10 min three times weekly for one or two consecutive weeks in male ob/ob mice. At 22 weeks of age, plasma lipid, glucose, other metabolites and relevant markers were measured by standard assays. Adipose tissue was assessed with immunohistochemical staining. Adipose tissue extracts were also analyzed with quantitative real-time polymerase chain reaction (Q-PCR) and Western blotting.
EA treatment is associated with decreased adipose tissue inflammation, and markedly decreased fat mass and adipocyte size in ob/ob mice. In obese mice, The protein levels of HIF-α were increased, EA shown a marked trend in inhibiting the hypoxic response in adipose tissue. The expression level of hypoxia-related genes (vascular endothelial growth factor A, VEGFA; glucose transporter type 1, Slc2al; glutathione peroxidase 1, GPX1) and inflammation-related genes (TNF-α, IL-6, MCP-1) expression were also reduced in adipose tissue after EA treatment. EA treatment decreased the macrophage recruitment and infiltration (F4/80), and in addition we found that decrease in NF-κB and increase in IkBα were both correlated to reduction in inflammatory processes in adipose tissue. This phenomenon was paralleled by the decrease in the levels of inflammatory cytokines, such as TNF-α, IL-6 and IL-1β in obese mice.
We conclude that EA prevents weight gain through modulation of HIF-1α-dependent pathways and inflammatory response in obese adipose tissues.
电针（EA）具有抗炎及多种与代谢途径相互作用的多效性作用。在本研究中，我们检验了以下假设：电针通过调节白色脂肪组织中缺氧诱导因子-1α（HIF1-α）依赖的途径，预防肥胖小鼠的炎症反应和体重增加。
将小鼠分为4组：非肥胖组、ob/ob组、接受3次治疗的ob/ob组、接受7次治疗的ob/ob组。对雄性ob/ob小鼠，每周3次，每次10分钟，在足三里（ST36）穴位施加低频电针（2赫兹），连续1周或2周。在22周龄时，通过标准检测方法测量血浆脂质、葡萄糖、其他代谢物及相关标志物。用免疫组织化学染色评估脂肪组织。还用定量实时聚合酶链反应（Q-PCR）和蛋白质免疫印迹法分析脂肪组织提取物。
电针治疗与ob/ob小鼠脂肪组织炎症减轻、脂肪量和脂肪细胞大小显著减小有关。在肥胖小鼠中，HIF-α蛋白水平升高，电针显示出抑制脂肪组织缺氧反应的明显趋势。电针治疗后，脂肪组织中缺氧相关基因（血管内皮生长因子A，VEGFA；葡萄糖转运蛋白1型，Slc2al；谷胱甘肽过氧化物酶1，GPX1）和炎症相关基因（TNF-α、IL-6、MCP-1）的表达水平也降低。电针治疗减少了巨噬细胞募集和浸润（F4/80），此外我们发现NF-κB的减少和IkBα的增加均与脂肪组织炎症过程的减轻相关。肥胖小鼠中炎症细胞因子如TNF-α、IL-6和IL-1β水平的降低与这一现象平行。
我们得出结论，电针通过调节肥胖脂肪组织中HIF-1α依赖的途径和炎症反应来预防体重增加。
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