Chen Qi, Li Zhiliang, Liu Bei, Fu Qiang, Yan Quanneng
Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Dec;35(12):1725-8.
To investigate the protective effects of Zhenwutang (ZWT) on cardiac function in mice with uremic cardiomyopathy (UCM).
Thirty C57BL/6 mice were randomized into 3 equal groups, including a sham-operated group, subtotal nephrectomy (UCM model) group and subtotal nephrectomy with ZWT treatment group. The mice in the former two groups were treated with distilled water. The changes in cardiac functions, myocardial structure and renal function of the mice were evaluated with echocardiography, HE staining and biochemical assay, respectively. Western blotting was used to detect the expression level of adenosine monophosphate-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) in the heart tissues.
Compared with the sham-operated group, the mice in the model group showed significantly lowered body weight and increased heart weight, heart index, left ventricular posterior wall thickness in diastole (LVPWd) and in systole (LVPWs), blood urea nitrogen (BUN) and serum creatinine (Scr) (P<0.05); Pathological examination revealed myocardial hypertrophy in the model group with markedly decreased expression levels of p-AMPK and significantly increased p-mTOR expression (P<0.05). ZWT treatment significantly decreased the heart weight, heart index, LVPWd, and LVPWs and expression level of p-mTOR (P<0.05), increased the expression level of p-AMPK (P<0.05), and obviously ameliorated histological injury of the heart in mice with UCM.
ZWT can protect the cardiac function in mice with subtotal nephrectomy-induced UCM possibly via the AMPK-mTOR signal pathway.
探讨真武汤（ZWT）对尿毒症心肌病（UCM）小鼠心功能的保护作用。
将30只C57BL/6小鼠随机分为3组，每组10只，分别为假手术组、次全肾切除（UCM模型）组和次全肾切除加ZWT治疗组。前两组小鼠给予蒸馏水。分别采用超声心动图、HE染色和生化检测评估小鼠的心功能、心肌结构和肾功能变化。采用蛋白质免疫印迹法检测心脏组织中腺苷酸活化蛋白激酶（AMPK）和雷帕霉素靶蛋白（mTOR）的表达水平。
与假手术组相比，模型组小鼠体重显著降低，心脏重量、心脏指数、舒张末期左心室后壁厚度（LVPWd）、收缩末期左心室后壁厚度（LVPWs）、血尿素氮（BUN）和血清肌酐（Scr）显著升高（P<0.05）；病理检查显示模型组心肌肥厚，p-AMPK表达水平显著降低，p-mTOR表达显著升高（P<0.05）。ZWT治疗显著降低了心脏重量、心脏指数、LVPWd和LVPWs以及p-mTOR的表达水平（P<0.05），增加了p-AMPK的表达水平（P<0.05），并明显改善了UCM小鼠的心脏组织学损伤。
真武汤可能通过AMPK-mTOR信号通路保护次全肾切除诱导的UCM小鼠的心功能。
