Ling Xuguang, Lou Anni, Li Yang, Yang Renqiang, Ning Zuowei, Li Xu
Department of Emergency Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Dec;35(12):1739-44.
To investigate the effect of losartan in regulating oxidative stress and the underlying mechanism in mice with ventilator-induced lung injury.
Thirty-six male C57 mice were randomly divided into control group, losartan treatment group, mechanical ventilation model group, and ventilation plus losartan treatment group. After the corresponding treatments, the lung injuries in each group were examined and the expressions of caveolin-1 and NOX4 in the lung tissues were detected.
The mean Smith score of lung injury was significantly higher in mechanical ventilation model group (3.3) than in the control group (0.4), and losartan treatment group (0.3); the mean score was significantly lowered in ventilation plus losartan treatment group (2.3) compared with that in the model group (P<0.05). The expressions of caveolin-1 and NOX4 were significantly higher in the model group than in the control and losartan treatment groups (P<0.05) but was obviously lowered after losartan treatment (P<0.05). Co-expression of caveolin-1 and NOX4 in the lungs was observed in the model group, and was significantly decreased after losartan treatment.
Losartan can alleviate ventilator-induced lung injury in mice and inhibit the expression of caveolin-1 and NOX4 and their interaction in the lungs.
探讨氯沙坦对呼吸机诱导的小鼠肺损伤中氧化应激的调节作用及其潜在机制。
将36只雄性C57小鼠随机分为对照组、氯沙坦治疗组、机械通气模型组和通气加氯沙坦治疗组。经过相应处理后，检测每组小鼠的肺损伤情况，并检测肺组织中小窝蛋白-1（caveolin-1）和NADPH氧化酶4（NOX4）的表达。
机械通气模型组的肺损伤平均史密斯评分（3.3）显著高于对照组（0.4）和氯沙坦治疗组（0.3）；通气加氯沙坦治疗组的平均评分（2.3）与模型组相比显著降低（P<0.05）。模型组中caveolin-1和NOX4的表达显著高于对照组和氯沙坦治疗组（P<0.05），但氯沙坦治疗后明显降低（P<0.05）。模型组肺组织中观察到caveolin-1和NOX4的共表达，氯沙坦治疗后显著减少。
氯沙坦可减轻小鼠呼吸机诱导的肺损伤，并抑制肺组织中caveolin-1和NOX4的表达及其相互作用。
