Perini Júlia Luiza, Hentschke Vítor Scotta, Sonza Anelise, Dal Lago Pedro
Laboratory of Experimental Physiology, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.
Post-Graduation Program in Health Sciences, UFCSPA, Porto Alegre, RS, Brazil.
Lasers Med Sci. 2016 Feb;31(2):241-8. doi: 10.1007/s10103-015-1849-8. Epub 2015 Dec 29.
The use of low-level laser therapy (LLLT) represents a new intervention modality that has been explored to enhance exercise performance. The aim of this study was to evaluate the influence of LLLT (GaAIAs-850 nm) at different doses on VO2max and on exercise performance in rats. Male Wistar rats were divided into three groups: "placebo" rats (P-LLLT, n = 10), rats at a dose of 0.315 J per treatment point of LLLT (8.7 J/cm(2)-LLLT, n = 10), and rats at a dose of 2.205 J per treatment point of LLLT (61.2 J/cm(2)-LLLT, n = 10). The LLLT was applied bilaterally at the biceps femoris, gluteus, lateral and medial gastrocnemius, iliopsoas, and adductor longus muscles. One spot in each muscle belly was applied, with a sum of 12 spots in each rat, once a day, for 10 days. All animals performed the maximal exercise test (ET) at a metabolic treadmill for rats, with simultaneous gas analysis. The distance covered was measured during ET, before and after the conclusion of the LLLT protocol. The data were compared by a repeated measures two-way ANOVA followed by the Student-Newman-Keuls post hoc tests (p < .05). The 61.2 J/cm(2)-LLLT group increased VO2basal (40 %), VO2max (24 %), VCO2max (17 %), and distance covered (34 %) after LLLT application on the skeletal muscle. No significant results were found comparing before and after conditions for the studied variables considering P-LLLT and 8.7 J/cm(2)-LLLT groups. The LLLT promoted in a dose-dependent manner an increase in oxygen consumption uptake and a performance increment of male Wistar rats.
低强度激光疗法（LLLT）的应用代表了一种新的干预方式，已被用于提高运动表现。本研究的目的是评估不同剂量的LLLT（GaAIAs - 850纳米）对大鼠最大摄氧量（VO2max）和运动表现的影响。雄性Wistar大鼠被分为三组：“安慰剂”组大鼠（P - LLLT，n = 10）、每次治疗点剂量为0.315焦耳的LLLT组大鼠（8.7焦耳/平方厘米 - LLLT，n = 10）和每次治疗点剂量为2.205焦耳的LLLT组大鼠（61.2焦耳/平方厘米 - LLLT，n = 10）。LLLT双侧应用于股二头肌、臀肌、腓肠肌外侧和内侧、髂腰肌以及长收肌。在每块肌腹上施加一个点，每只大鼠共12个点，每天一次，持续10天。所有动物在大鼠代谢跑步机上进行最大运动测试（ET），同时进行气体分析。在ET期间以及LLLT方案结束前后测量所跑距离。数据通过重复测量双向方差分析，随后进行Student - Newman - Keuls事后检验（p < 0.05）。在骨骼肌上应用LLLT后，61.2焦耳/平方厘米 - LLLT组的基础摄氧量（VO2basal）增加了约40%，最大摄氧量（VO2max）增加了约24%，最大二氧化碳排出量（VCO2max）增加了约17%，所跑距离增加了约34%。对于P - LLLT组和8.7焦耳/平方厘米 - LLLT组，比较研究变量前后的情况未发现显著结果。LLLT以剂量依赖的方式促进了雄性Wistar大鼠的耗氧量增加和运动表现提升。
