Veronica M, Ali Altaf, Venkateshwari A, Mamata D, Nallari Pratibha
Department of Genetics, University College of Science, Osmania University, Hyderabad, 500007, Telangana, India.
Institute of Genetics and Hospital for Genetic Disorders, Ameerpet, Hyderabad, Telangana, India.
Tumour Biol. 2016 Jun;37(6):8067-74. doi: 10.1007/s13277-015-4711-5. Epub 2015 Dec 29.
The steroid hormones estradiol and progesterone play an important role in the pathophysiology of fibroids that occurs in 20-25 % of women in the reproductive age. Our study examines the risk imposed by estrogen and progesterone plasma levels in correlation with the ERβ (-13950T/C) and PGR (+331G/A) receptor gene polymorphisms. The study population included 296 individuals (146 UL cases and 150 female controls). Hormonal levels were estimated by ELISA and genotyping was carried out by PCR-RFLP analysis, and the obtained results were statistically analyzed. Estrogen levels were found to be high in cases with the "TC" genotype of ERβ receptor polymorphism compared to controls, whereas individuals with "GA" and "AA" genotype of PGR receptor polymorphism showed high progesterone levels for cases when compared to controls. The TC genotype of the ERβ receptor polymorphism and the GA and AA genotypes of the PGR receptor polymorphism and their respective hormonal levels can be developed as markers in the prediction of uterine fibroids.
甾体激素雌二醇和孕酮在肌瘤的病理生理学中起着重要作用，肌瘤发生在20%至25%的育龄妇女中。我们的研究考察了雌激素和孕酮血浆水平与雌激素受体β（ERβ，-13950T/C）和孕激素受体（PGR，+331G/A）基因多态性相关的风险。研究人群包括296名个体（146例子宫平滑肌瘤患者和150名女性对照）。通过酶联免疫吸附测定法（ELISA）估计激素水平，通过聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）进行基因分型，并对所得结果进行统计学分析。与对照组相比，ERβ受体多态性为“TC”基因型的患者雌激素水平较高，而PGR受体多态性为“GA”和“AA”基因型的患者与对照组相比，其孕酮水平较高。ERβ受体多态性的“TC”基因型、PGR受体多态性的“GA”和“AA”基因型及其各自的激素水平可作为预测子宫肌瘤的标志物。
