Zhang Xiang, Wang Yanmin, Zhong Mingwei, Liu Teng, Han Haifeng, Zhang Guangyong, Liu Shaozhuang, Wei Meng, Wu Qunzheng, Hu Sanyuan
Department of General Surgery, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
Obes Surg. 2016 Aug;26(8):1890-9. doi: 10.1007/s11695-015-2031-x.
Bile acids (BAs) have emerged as important signaling molecules in regulating metabolism and are closely related to gut microbiota. Bariatric surgery elevates serum BAs and affects gut microbiota universally. However, the specific profiles of postsurgical BA components and gut microbiota are still controversial. The aim of this study is to investigate the serum profiles of BA components and gut microbiota after duodenal-jejunal bypass (DJB).
DJB and SHAM procedures were performed in a high-fat-diet/streptozotocin-induced diabetic rat model. Body weight, energy intake, oral glucose tolerance test, insulin tolerance test, HOMA-IR, serum hormones, serum BAs, expression of BA transporters, and gut microbiota were analyzed at week 2 and week 12 postsurgery.
Compared with SHAM, DJB achieved rapid and durable improvement in glucose tolerance and insulin sensitivity, with enhanced GLP-1 secretion. DJB also elevated serum BAs, especially the taurine-conjugated BAs, with upregulation of BA transporters in the terminal ileum. The phylum level of Firmicutes and Proteobacteria abundance was increased postsurgery, at the expense of Bacteroidetes.
DJB preferentially increases serum taurine-conjugated BAs, probably because of more BA reabsorption in the terminal ileum. The gut microbiota is altered with more Firmicutes and Proteobacteria and less Bacteroidetes.
胆汁酸（BAs）已成为调节代谢的重要信号分子，且与肠道微生物群密切相关。减肥手术可提高血清胆汁酸水平，并普遍影响肠道微生物群。然而，术后胆汁酸成分和肠道微生物群的具体特征仍存在争议。本研究旨在调查十二指肠空肠旁路术（DJB）后胆汁酸成分和肠道微生物群的血清特征。
在高脂饮食/链脲佐菌素诱导的糖尿病大鼠模型中进行DJB和假手术。在术后第2周和第12周分析体重、能量摄入、口服葡萄糖耐量试验、胰岛素耐量试验、HOMA-IR、血清激素、血清胆汁酸、胆汁酸转运体的表达以及肠道微生物群。
与假手术组相比，DJB使葡萄糖耐量和胰岛素敏感性迅速且持久地得到改善，同时增强了胰高血糖素样肽-1（GLP-1）的分泌。DJB还提高了血清胆汁酸水平，尤其是牛磺酸结合型胆汁酸，同时回肠末端胆汁酸转运体上调。术后厚壁菌门和变形菌门的丰度增加，拟杆菌门减少。
DJB优先增加血清牛磺酸结合型胆汁酸，可能是因为回肠末端胆汁酸重吸收增加。肠道微生物群发生改变，厚壁菌门和变形菌门增多，拟杆菌门减少。
