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肢体远程缺血后处理可保护大鼠脑缺血免受与缺氧诱导因子-1α表达相关的损伤。

Limb remote ischemic postconditioning protects cerebral ischemia from injury associated with expression of HIF-1α in rats.

作者信息

Zong Yonghua, Jiang Ling, Zhang Mingxiao, Zhou Fangfang, Qi Wenqian, Li Shuai, Yang Huijun, Zou Yu, Xia Qingjie, Zhou Xue, Hu Xiaosong, Wang Tinghua

机构信息

Department of Morphology Lab and Department of Graduate, Chengdu Medical College, Sichuan, 610500, China.

Department of Anesthesiology and Institute of Neurological Disease, State Key Laboratory of Biotherapy, West China Hospital, Sichuan, 610041, China.

出版信息

BMC Neurosci. 2015 Dec 29;16:97. doi: 10.1186/s12868-015-0235-6.

DOI:10.1186/s12868-015-0235-6
PMID:26715469
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4696280/
Abstract

BACKGROUND

Limb remote ischemic postconditioning (LRIP) can ameliorate cerebral ischemia-reperfusion injury (IRI), while the underlying mechanism remains elusive. Hypoxia-inducible factor 1α (HIF-1α) is an important transcription factor during cerebral ischemia damage. However, whether the neuroprotective effect of LRIP could be associated with HIF-1α is somewhat unclear. Here we tested the hypothesis that Limb remote ischemic postconditioning (LRIP) protecting brain from injury in middle cerebral artery occlusion (MCAO) rat model was associated with HIF-1α expression.

RESULTS

LRIP was conducted with 3 cycles of 10 min occlusion/10 min reperfusion at the beginning of reperfusion. The analysis of neurobehavioral function and triphenyltetrazolium chloride (TTC) staining showed the neurological deficit, brain infarct and cerebral edema, caused by ischemia-reperfusion injury (IRI), were dramatically ameliorated in LRIP administrated animals. Meanwhile, the result of Q-PCR and western blot revealed that the overexpression of HIF-1α induced by IRI could be notably suppressed by LRIP treatment.

CONCLUSIONS

LRIP exhibits a protective effect against cerebral ischemia/reperfusion and the possible mechanism is associated with the suppression of HIF-1α in stroke rats.

摘要

背景

肢体远程缺血后处理（LRIP）可改善脑缺血再灌注损伤（IRI），但其潜在机制仍不清楚。缺氧诱导因子1α（HIF-1α）是脑缺血损伤过程中的一种重要转录因子。然而，LRIP的神经保护作用是否与HIF-1α有关尚不清楚。在此，我们检验了以下假设：在大脑中动脉闭塞（MCAO）大鼠模型中，肢体远程缺血后处理（LRIP）对脑损伤的保护作用与HIF-1α表达有关。

结果

在再灌注开始时进行3个周期的10分钟闭塞/10分钟再灌注的LRIP处理。神经行为功能分析和氯化三苯基四氮唑（TTC）染色显示，LRIP处理的动物中，由缺血再灌注损伤（IRI）引起的神经功能缺损、脑梗死和脑水肿均得到显著改善。同时，Q-PCR和蛋白质免疫印迹结果显示，LRIP处理可显著抑制IRI诱导的HIF-1α过表达。

结论

LRIP对脑缺血/再灌注具有保护作用，其可能机制与抑制中风大鼠的HIF-1α有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/3f20b5165e82/12868_2015_235_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/8638e34a5648/12868_2015_235_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/718276292e0d/12868_2015_235_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/d79ea283dba5/12868_2015_235_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/9de5ab9e9637/12868_2015_235_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/3f20b5165e82/12868_2015_235_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/8638e34a5648/12868_2015_235_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/718276292e0d/12868_2015_235_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/d79ea283dba5/12868_2015_235_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/9de5ab9e9637/12868_2015_235_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01e8/4696280/3f20b5165e82/12868_2015_235_Fig5_HTML.jpg

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